macho-1-related genes in Ciona embryos
macho-1-related genes in Ciona embryos
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Ciona 胚胎中的 macho-1 相关基因
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
N. Satoh
中科院分区：
文献类型：
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作者：
Y. Satou;K. Yagi;K. Imai;Lixy Yamada;H. Nishida;N. Satoh
抽象的。在海鞘中，位于卵肌质中的母体因子对于幼虫肌细胞的特化和随后的分化是必不可少的。Halocynthia roretzi的macho-1基因编码锌指蛋白：该基因仅在母体中表达，所产生的母体mRNA定位于肌浆，并且该基因功能对于肌细胞分化是必需的。在这里，我们的特点是macho-1同系物，Ci-macho 1的玻璃海鞘和Cs-macho 1的玻璃海鞘savignyi。有趣的是，我们发现Cionamacho-1基因在母体和合子中表达：它们的母体转录本定位于肌浆，而它们的合子表达在中枢神经系统细胞神经胚形成后可见。用吗啉代反义寡核苷酸抑制Cs-Macho 1的功能导致抑制肌肉特异性肌动蛋白基因合子表达的起始。我们提出了一个可能的进化方案，其中一个祖先Zic相关基因引起了两个macho-1样肌肉决定基因以及神经元Zic基因。
Abstract. In ascidians, maternal factor(s) localized in the myoplasm of the egg are essential for specification and subsequent differentiation of larval muscle cells. The macho-1 gene of Halocynthia roretzi encodes a zinc-finger protein: the gene is only expressed maternally, the resultant maternal mRNA is localized to the myoplasm, and the gene function is essential for muscle cell differentiation. Here we have characterized macho-1 homologues, Ci-macho1 of Ciona intestinalis and Cs-macho1 of Ciona savignyi. Interestingly, we found that the Cionamacho-1 genes are expressed both maternally and zygotically: their maternal transcript is localized to the myoplasm while their zygotic expression is seen after neurulation in cells of the central nervous system. Functional suppression of Cs-macho1 with morpholino antisense oligonucleotide resulted in inhibition of the initiation of zygotic expression of a muscle-specific actin gene. We propose a possible evolutionary scenario in which an ancestral Zic-related gene gave rise to both the macho-1-like muscle determinant gene as well as neuronal Zic genes.
影响因子：
10.5
作者：
BENEDYK, MJ;MULLEN, JR;DINARDO, S
通讯作者：
DINARDO, S