Acute and chronic dose/response effect of benzene inhalation on the peripheral blood, bone marrow, and spleen cells of CD-1 male mice.
Acute and chronic dose/response effect of benzene inhalation on the peripheral blood, bone marrow, and spleen cells of CD-1 male mice.
复制标题
吸入苯对CD-1雄性小鼠外周血、骨髓和脾细胞的急性和慢性剂量/反应影响。
DOI：
10.1016/0041-008x(81)90191-5
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发表时间：
1981
影响因子：
3.8
通讯作者：
R. Albert
中科院分区：
文献类型：
--
作者：
J. D. Green;C. Snyder;J. Lobue;B. Goldstein;R. Albert
研究了雄性CD-1小鼠吸入苯血液毒性以识别干细胞后代，这些小鼠暴露于以下平均苯浓度：1.1、9.9、103、306、603、1276、2416和4862 ppm，时间为6小时/天× 5天。其他组小鼠暴露6小时/天× 5天/周× 10周至9.6 ppm，或6小时/天× 5天/周× 26周至302 ppm。暴露5天后，粒细胞减少症和淋巴细胞减少症在浓度≥103 ppm时被观察到，WBC差异没有变化。RBC计数仅在两个最高暴露水平时被抑制，而红细胞比容受到不同程度的影响，没有明显的剂量/反应效应。≥103 ppm时，骨髓和脾细胞性均降低。骨髓淋巴细胞、脾淋巴细胞和骨髓粒细胞随着总细胞量的减少而减少，但几乎在所有暴露水平下，脾粒细胞和脾脏重量都受到抑制。≥103 ppm时，骨髓和脾脏的有核红细胞几乎全部被抑制。在9.6 ppm的苯环境中暴露50天，总剂量相当于在103 ppm环境中暴露5天，在外周血或骨髓中未引起可检测到的变化，但观察到脾重量和细胞数量增加。接触302ppm苯26周导致淋巴细胞减少症和贫血。骨髓细胞减少到对照组的32%，主要是由于淋巴细胞和粒细胞减少。脾脏细胞数和重量分别减少到对照组的17%和67%。脾脏细胞减少主要是由于淋巴细胞减少到对照组的5%。长期暴露于302ppm苯中导致外周血、骨髓和脾脏细胞形态不典型。
Inhaled benzene hematotoxicity to recognize stem cell progeny was studied in male CD-1 mice exposed for 6 hr/day × 5 days to one of the following mean benzene concentrations: 1.1, 9.9, 103, 306, 603, 1276, 2416, and 4862 ppm. Additional groups of mice were exposed for 6 hr/day × 5 days/week × 10 weeks to 9.6 ppm, or 6 hr/day × 5 days/week × 26 weeks to 302 ppm. Following the 5-day exposures, granulocytopenia and lymphocytopenia were observed at levels ≥ 103 ppm with no change in WBC differential. RBC counts were depressed only at the two highest exposure levels while hematocrits were variably affected and showed no clear dose/response effect. Marrow and splenic cellularities were reduced at all levels ≥ 103 ppm. Marrow lymphocytes, splenic lymphocytes, and marrow granulocytes were reduced in accordance with the reduction in total cellularity, however, splenic granulocytes and spleen weights were depressed at almost all exposure levels. Nucleated RBCs in the marrow and spleen were depressed at almost all levels ≥ 103 ppm. Exposure for 50 days to 9.6 ppm benzene, a total dose equivalent to that delivered over 5 days at 103 ppm, induced no detectable changes in the peripheral blood or bone marrow, however, increases in splenic weight and cellularity were observed. Twenty-six weeks of exposure to 302 ppm benzene resulted in lymphocytopenia and anemia. Marrow cellularity was reduced to 32% of control and was due primarily to a reduction in lymphocytes and granulocytes. Spleen cellularity and weight were reduced to 17 and 67% of control, respectively. Decreased spleen cellularity was due primarily to a reduction in lymphocytes to 5% of control. These extended exposures to 302 ppm benzene resulted in atypical cell morphology in the peripheral blood, bone marrow, and spleen.
影响因子：
3.8
作者：
C. Snyder;B. Goldstein;A. Sellakumar;Isabel Bromberg;S. Laskin;Roy E. Albert
通讯作者：
C. Snyder;B. Goldstein;A. Sellakumar;Isabel Bromberg;S. Laskin;Roy E. Albert