A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits.
A phylogeny and molecular barcodes for Caenorhabditis, with numerous new species from rotting fruits.
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DOI：
10.1186/1471-2148-11-339
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发表时间：
2011-11-21
影响因子：
3.4
通讯作者：
Fitch DH
中科院分区：
文献类型：
--
作者：
Kiontke KC;Félix MA;Ailion M;Rockman MV;Braendle C;Pénigault JB;Fitch DH
秀丽隐杆线虫是生物学中的主要实验室模型。在本研究开始时，培养物中仅可获得10种小杆线虫。其中许多，如C。线虫，大多数是从人工堆肥堆中分离出来的，它们更自然的栖息地是未知的。在腐烂的果实、花和茎中发现了小杆线虫的繁殖。通过收集世界各地的大量样本，发现了16个新的小杆线虫物种。我们进行了交配试验，以建立生物物种的地位，并发现了一些情况下，半可育或不育的杂交后代。我们使用ITS 2 rDNA序列建立了所有物种的条形码。通过获得两个rRNA和9个蛋白质编码基因的序列数据，我们确定了26个物种在文化中可能的系统发育关系。新物种是两个清晰的姐妹分支的一部分，我们称之为Elegans超级群体和Drosophilae超级群体。进一步对繁殖方式、交配行为和雄尾形态等表型性状进行评分，并讨论了它们与生殖发育的一致性。在Elegans超类群的茎种中，第2条和第3条射线之间的小空间进化了一次;在C. angaria，C. sp.8和C. sp. 12.其他几个性格变化也是同时发生的。例如，雌雄同体在C. elegans、秀丽隐杆线虫C. briggsae和C. sp. 11.几个物种可以同时出现在同一个地方，甚至同一个水果。在全球范围内，有些种类具有世界性分布：C。briggsae分布特别广，而C. elegans和C. remanei主要分布于温带地区，C. brenneri和C. sp. 11专门在热带地区。其他物种分布有限，例如C。sp.5似乎仅限于中国，C. sp.7分布于西非，C. 8、美国东部。小杆线虫是“水果蠕虫”，而不是土壤线虫。这16个新种提供了一个资源，它们的亲缘关系为进一步研究基因组和表型性状的进化提供了一个框架。
The nematode Caenorhabditis elegans is a major laboratory model in biology. Only ten Caenorhabditis species were available in culture at the onset of this study. Many of them, like C. elegans, were mostly isolated from artificial compost heaps, and their more natural habitat was unknown. Caenorhabditis nematodes were found to be proliferating in rotten fruits, flowers and stems. By collecting a large worldwide set of such samples, 16 new Caenorhabditis species were discovered. We performed mating tests to establish biological species status and found some instances of semi-fertile or sterile hybrid progeny. We established barcodes for all species using ITS2 rDNA sequences. By obtaining sequence data for two rRNA and nine protein-coding genes, we determined the likely phylogenetic relationships among the 26 species in culture. The new species are part of two well-resolved sister clades that we call the Elegans super-group and the Drosophilae super-group. We further scored phenotypic characters such as reproductive mode, mating behavior and male tail morphology, and discuss their congruence with the phylogeny. A small space between rays 2 and 3 evolved once in the stem species of the Elegans super-group; a narrow fan and spiral copulation evolved once in the stem species of C. angaria, C. sp. 8 and C. sp. 12. Several other character changes occurred convergently. For example, hermaphroditism evolved three times independently in C. elegans, C. briggsae and C. sp. 11. Several species can co-occur in the same location or even the same fruit. At the global level, some species have a cosmopolitan distribution: C. briggsae is particularly widespread, while C. elegans and C. remanei are found mostly or exclusively in temperate regions, and C. brenneri and C. sp. 11 exclusively in tropical zones. Other species have limited distributions, for example C. sp. 5 appears to be restricted to China, C. sp. 7 to West Africa and C. sp. 8 to the Eastern United States. Caenorhabditis are "fruit worms", not soil nematodes. The 16 new species provide a resource and their phylogeny offers a framework for further studies into the evolution of genomic and phenotypic characters.
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影响因子：
11.8
作者：
Hill, RC;de Carvalho, CE;Haag, ES
通讯作者：
Haag, ES
影响因子：
4.9
作者：
Cutter, Asher D.;Yan, Weiang;Felix, Marie-Anne
通讯作者：
Felix, Marie-Anne
影响因子：
3.3
作者：
Cutter, Asher D.;Wasmuth, James D.;Washington, Nicole L.
通讯作者：
Washington, Nicole L.
DOI：
10.1098/rspb.2002.1996
发表时间：
2002-06-07
影响因子：
4.7
作者：
LaMunyon, CW;Ward, S
通讯作者：
Ward, S
影响因子：
2.7
作者：
Brauchle, Michael;Kiontke, Karin;Piano, Fabio
通讯作者：
Piano, Fabio