Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating.
Direct allele introgression into pure chicken breeds using Sire Dam Surrogate (SDS) mating.
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DOI：
10.1038/s41467-020-20812-x
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发表时间：
2021-01-28
影响因子：
16.6
通讯作者：
McGrew MJ
中科院分区：
文献类型：
--
作者：
Ballantyne M;Woodcock M;Doddamani D;Hu T;Taylor L;Hawken RJ;McGrew MJ
家禽是最丰富的牲畜物种，全球每年饲养超过600亿只鸡。大多数鸡是从商业鸡群中生产的，但许多本地鸡种在农村经济中发挥着重要作用，因为它们非常适应当地的环境和食腐条件。在鸡中进行精确遗传改变的能力将允许验证负责气候适应和疾病恢复的遗传变异，以及在品种之间转移有益等位基因。在这里，我们产生了一种新的诱导不育的代理宿主鸡。将供体基因组编辑的原始生殖细胞引入不育的雄性和雌性宿主胚胎中产生仅携带外源生殖细胞的成年鸡。随后直接交配的替代宿主，Sire Dam Surrogate（SDS）交配，重建了供体鸡品种携带编辑的等位基因在一个单一的一代。我们证明了渐渗和验证的两个羽毛性状的等位基因，显性白色和卷曲到两个纯粹的鸡品种使用SDS替代主机。鸡是一种重要的农业动物，也是一种生物模式动物。在这里，作者证明了纯种基因组编辑的小鸡可以通过携带供体基因组编辑的生殖细胞的无菌替代宿主的直接交配来生产任何鸡品种。
Poultry is the most abundant livestock species with over 60 billion chickens raised globally per year. The majority of chicken are produced from commercial flocks, however many indigenous chicken breeds play an important role in rural economies as they are well adapted to local environmental and scavenging conditions. The ability to make precise genetic changes in chicken will permit the validation of genetic variants responsible for climate adaptation and disease resilience, and the transfer of beneficial alleles between breeds. Here, we generate a novel inducibly sterile surrogate host chicken. Introducing donor genome edited primordial germ cells into the sterile male and female host embryos produces adult chicken carrying only exogenous germ cells. Subsequent direct mating of the surrogate hosts, Sire Dam Surrogate (SDS) mating, recreates the donor chicken breed carrying the edited allele in a single generation. We demonstrate the introgression and validation of two feather trait alleles, Dominant white and Frizzle into two pure chicken breeds using the SDS surrogate hosts. Chicken are a biological model and an important agricultural animal. Here, the authors demonstrate that pure breed genome edited chicks can be produced for any chicken breed by direct mating of sterile surrogate hosts carrying donor genome edited germ cells.
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影响因子：
4.6
作者：
Idoko-Akoh A;Taylor L;Sang HM;McGrew MJ
通讯作者：
McGrew MJ
DOI：
10.1073/pnas.2010102117
发表时间：
2020-09-29
影响因子：
11.1
作者：
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影响因子：
4.8
作者：
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通讯作者：
Park, Tae Sub
影响因子：
1.8
作者：
Kito, Gakushi;Aramaki, Shinya;Hattori, Masa-aki
通讯作者：
Hattori, Masa-aki
影响因子：
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作者：
Marin, Virna;Cribioli, Elisabetta;Pule, Martin
通讯作者：
Pule, Martin