Arrest of Root Carious Lesions via Sodium Fluoride, Chlorhexidine and Silver Diamine Fluoride In Vitro.
Arrest of Root Carious Lesions via Sodium Fluoride, Chlorhexidine and Silver Diamine Fluoride In Vitro.
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DOI：
10.3390/ma11010009
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发表时间：
2017-12-22
期刊：
影响因子：
--
通讯作者：
Schwendicke F
中科院分区：
文献类型：
--
作者：
Göstemeyer G;Schulze F;Paris S;Schwendicke F
目的：目的比较洗必泰（CHX）、银二胺氟化物（SDF）保护漆和（或）氟化钠（NaF）冲洗液对根面龋的抑制作用。背景资料：需要用于治疗根龋病变的有效且易于应用的干预，因为这些病变在老年个体中非常普遍。研究方法：在100个牛牙本质样本中，使用乙酸和连续培养的鼠李糖乳杆菌生物膜模型诱导人工根龋病变。每个诱导的损伤的四分之一被挖掘，并且基线牙本质细菌计数被评估为菌落形成单位（CFU）/mg。将样品分配至四种处理中的一种（n = 25/组）：（1）未处理的对照;（2）38%SDF或（3）35%CHX清漆，每种施用一次，在随后的病变进展阶段加每日500 ppm NaF冲洗;和（4）每日仅NaF冲洗。将样品重新转移到生物膜模型中并进行致龋攻击。6天后，每个病变的另一个四分之一用于评估细菌计数，剩余样品用于使用显微放射照相术评估综合矿物质损失（ΔZ）。结果如下：ΔZ在对照组（中位数（第25/75个样本）：9082（7859/9782）vol % × µm）、NaF组（6704（4507/9574）和SDF 7206（5389/8082））之间无显著差异（p < 0.05/Kruskal-Wallis检验）。与所有其他组相比，CHX显著降低ΔZ（3385（2447/4496））（p < 0.05）。对照组（1451（875/2644）CFU/µg）和NaF组（750（260/1401））之间的细菌数量无显著差异（p > 0.05）。与对照组相比，SDF显著降低了细菌计数（360（136/1166））（p < 0.05）。与NaF和对照相比，CHX显著降低细菌计数（190（73/517））（p < 0.05）。结论：CHX保护漆加氟化钠漱口液能有效抑制根面龋。
Objective: To compare the root carious lesion arrest of chlorhexidine (CHX) and silver diamine fluoride (SDF) varnishes and/or sodium fluoride rinses (NaF) in vitro. Background: Effective and easily applicable interventions for treating root carious lesions are needed, as these lesions are highly prevalent amongst elderly individuals. Methods: In 100 bovine dentin samples, artificial root carious lesions were induced using acetic acid and a continuous-culture Lactobacillus rhamnosus biofilm model. One quarter of each induced lesion was excavated and baseline dentinal bacterial counts assessed as Colony-Forming-Units (CFU) per mg. Samples were allocated to one of four treatments (n = 25/group): (1) untreated control; (2) 38% SDF or (3) 35% CHX varnish, each applied once, plus 500 ppm daily NaF rinse in the subsequent lesion progression phase; and (4) daily NaF rinses only. Samples were re-transferred to the biofilm model and submitted to a cariogenic challenge. After six days, another quarter of each lesion was used to assess bacterial counts and the remaining sample was used to assess integrated mineral loss (ΔZ) using microradiography. Results: ΔZ did not differ significantly between control (median (25th/75th percentiles): 9082 (7859/9782) vol % × µm), NaF (6704 (4507/9574) and SDF 7206 (5389/8082)) (p < 0.05/Kruskal–Wallis test). CHX significantly reduced ΔZ (3385 (2447/4496)) compared with all other groups (p < 0.05). Bacterial numbers did not differ significantly between control (1451 (875/2644) CFU/µg) and NaF (750 (260/1401)) (p > 0.05). SDF reduced bacterial counts (360 (136/1166)) significantly compared with control (p < 0.05). CHX reduced bacterial counts (190 (73/517)) significantly compared with NaF and control (p < 0.05). Conclusion: CHX varnish plus regular NaF rinses arrested root carious lesions most successfully.
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影响因子：
4.2
作者：
Schwendicke, F.;Doerfer, C.;Paris, S.
通讯作者：
Paris, S.
影响因子：
12.7
作者：
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影响因子：
4.2
作者：
BUSKES, JAKM;CHRISTOFFERSEN, J;ARENDS, J
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影响因子：
4.2
作者：
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影响因子：
2.3
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