Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries.
Large conductance Ca2+-activated K+ channels contribute to vascular function in nonpregnant human uterine arteries.
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DOI：
10.1177/1933719108319160
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发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
Liu XT
中科院分区：
文献类型：
--
作者：
Rosenfeld CR;Word RA;DeSpain K;Liu XT
大电导钾通道(BKCa)表达于非妊娠和妊娠绵羊子宫动脉(UA)的平滑肌，参与基础血管张力的调节以及对雌激素和血管紧张剂的反应。为了确定BKCa是否在女性中表达并参与尿酸功能，我们在择期子宫切除术中收集了非妊娠妇女(n=31)的尿酸，并分析了亚单位蛋白、免疫组织化学定位和内皮剥离环的功能。UA表达BKCaα-，β1-和β2-亚基蛋白。KCL和苯肾上腺素(PE，一种α1激动剂)可引起剂量依赖性的血管收缩(P<；0.001)，而预先收缩PE的UA可被硝普钠(SNP；P<；0.001)剂量依赖性地松弛。BKCa抑制剂四乙基氯化铵(TEA，0.004.2~1.0 mM)可剂量依赖性地增加静息张力(P=0.0 5；1 mM时为2 8±5.3%)，增强PE(10−6 M)引起的血管收缩(P<；0.0 4)，并减弱硝普钠在1 mM时的松弛作用(P=0.0 2)。BKCa在人类不稳定性心绞痛(UA)中表达，通过减弱血管收缩反应和促进一氧化氮诱导的血管松弛来调节血管功能。
Large conductance K+ channels (BKCa) are expressed in uterine artery (UA) smooth muscle from nonpregnant and pregnant sheep and contribute to the regulation of basal vascular tone and responses to estrogen and vasoconstrictors. To determine if BKCa are expressed in women and contribute to UA function, we collected UA from nonpregnant women (n=31) at elective hysterectomy and analyzed for subunit protein, localization with immunohistochemistry and function using endothelium-denuded rings. UA expresses BKCa α-, β1- and β2-subunit protein. KCl and phenylephrine (PE, an α1-agonist) caused dose-dependent vasoconstriction (P<0.001), and UA precontracted with PE dose-dependently relaxed with sodium nitroprusside (SNP; P<0.001). Tetraethylammonium chloride (TEA, 0.2–1.0 mM), a BKCa inhibitor, dose-dependently increased resting tone (P=0.004; 28±5.3% with 1.0 mM), enhanced PE-induced (10−6 M) vasoconstriction (P<0.04), and attenuated SNP-induced relaxation at 1.0 mM (P=0.02). BKCa are expressed in human UA and modulate vascular function by attenuating vasoconstrictor responses and contributing to nitric oxide-induced vasorelaxation.
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DOI：
10.1152/nips.01387.2002
发表时间：
2002-08-01
期刊：
NEWS IN PHYSIOLOGICAL SCIENCES
影响因子：
--
作者：
Orio, P;Rojas, P;Latorre, R
通讯作者：
Latorre, R
影响因子：
5.5
作者：
Nishimaru, K;Eghbali, M;Toro, L
通讯作者：
Toro, L
影响因子：
4.1
作者：
Holdiman, AJ;Fergus, DJ;England, SK
通讯作者：
England, SK
DOI：
10.1152/ajpregu.00851.2006
发表时间：
2007-07-01
影响因子：
2.8
作者：
Hutanu, Catalina;Cox, Blair E.;Rosenfeld, Charles R.
通讯作者：
Rosenfeld, Charles R.
影响因子：
3.8
作者：
Orio, P;Latorre, R
通讯作者：
Latorre, R