Antigravitropic PIN polarization maintains non-vertical growth in lateral roots.
Antigravitropic PIN polarization maintains non-vertical growth in lateral roots.
复制标题
DOI：
10.1038/s41477-023-01478-x
复制
发表时间：
2023-09
期刊：
影响因子：
18
通讯作者：
Kepinski, Stefan
中科院分区：
文献类型：
--
作者：
Roychoudhry, Suruchi;Sageman-Furnas, Katelyn;Wolverton, Chris;Grones, Peter;Tan, Shutang;Molnar, Gergely;De Angelis, Martina;Goodman, Heather L.;Capstaff, Nicola;Lloyd, James P. B.;Mullen, Jack;Hangarter, Roger;Friml, Jiri;Kepinski, Stefan
侧根通常保持在相对于重力的非垂直角。这些向重力的设定点角很耐人寻味，因为它们的维持要求根系能够在重力矢量的作用下和在重力矢量的作用下影响生长反应，这一现象以前被归因于引力向量力与反重力偏移量机制的作用。在这里，我们展示了垂直主根中调节向重力性的成分--作用于它们的PIN和磷酸酶--是如何在它们的调节中重新配置的，以便在一定角度范围内保持侧根的生长。我们表明，拟南芥侧根向下和向上弯曲的能力需要生长素不对称的产生，并且是由向下的重力生长素通量相对于与角度无关的向上的反重力生长素通量的角度依赖的变化所驱动的。此外，我们证明了生长素在侧根中的对称性分布在重力设定点角，这可以追溯到小柱中PIN3和PIN7生长素转运体的净、平衡极化。这些生长素通量通过改变小柱中PIN蛋白的磷调节来改变，要么是通过引入PIN3磷酸变异体，要么是通过操纵磷酸酶亚单位PP2A/RCN1的水平。最后，我们发现生长素除了驱动侧根的定向生长外，还通过增加RCN1水平在侧根柱体内诱导更多的垂直生长，导致PIN3定位下移，从而减少向上的反重力生长素通量的幅度。侧根在重力作用下以特定角度生长。重力感应细胞中的两种生长素转运蛋白PIN3和PIN7建立了激素梯度，平衡向下和向上的生长，以产生稳定的、非垂直的根。
Lateral roots are typically maintained at non-vertical angles with respect to gravity. These gravitropic setpoint angles are intriguing because their maintenance requires that roots are able to effect growth response both with and against the gravity vector, a phenomenon previously attributed to gravitropism acting against an antigravitropic offset mechanism. Here we show how the components mediating gravitropism in the vertical primary root—PINs and phosphatases acting upon them—are reconfigured in their regulation such that lateral root growth at a range of angles can be maintained. We show that the ability of Arabidopsis lateral roots to bend both downward and upward requires the generation of auxin asymmetries and is driven by angle-dependent variation in downward gravitropic auxin flux acting against angle-independent upward, antigravitropic flux. Further, we demonstrate a symmetry in auxin distribution in lateral roots at gravitropic setpoint angle that can be traced back to a net, balanced polarization of PIN3 and PIN7 auxin transporters in the columella. These auxin fluxes are shifted by altering PIN protein phosphoregulation in the columella, either by introducing PIN3 phosphovariant versions or via manipulation of levels of the phosphatase subunit PP2A/RCN1. Finally, we show that auxin, in addition to driving lateral root directional growth, acts within the lateral root columella to induce more vertical growth by increasing RCN1 levels, causing a downward shift in PIN3 localization, thereby diminishing the magnitude of the upward, antigravitropic auxin flux. Lateral roots grow at specific angles with respect to gravity. Two auxin transporters, PIN3 and PIN7, in gravi-sensing cells establish hormone gradients that balance downward and upward growth to generate stable, non-vertical roots.
登录
查看更多内容
影响因子：
4.6
作者：
Grones P;Abas M;Hajný J;Jones A;Waidmann S;Kleine-Vehn J;Friml J
通讯作者：
Friml J
DOI：
10.1016/s0273-1177(03)00249-7
发表时间：
2003-01-01
期刊：
SPACE LIFE SCIENCES: GRAVITY-RELATED PROCESSES IN PLANTS
影响因子：
--
作者：
Mullen, JL;Hangarter, RP
通讯作者：
Hangarter, RP
影响因子：
18
作者：
Glanc, Matous;Fendrych, Matyas;Friml, Jiri
通讯作者：
Friml, Jiri
影响因子：
18
作者：
Ge, Liangfa;Chen, Rujin
通讯作者：
Chen, Rujin
DOI：
10.1073/pnas.2020857118
发表时间：
2021-01-05
影响因子：
11.1
作者：
Abas, Lindy;Kolb, Martina;Hammes, Ulrich Z.
通讯作者：
Hammes, Ulrich Z.