Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion.
Quantitative analysis of cadherin-catenin-actin reorganization during development of cell-cell adhesion.
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DOI：
10.1083/jcb.135.6.1899
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发表时间：
1996-12
影响因子：
7.8
通讯作者：
Smith, SJ
中科院分区：
文献类型：
--
作者：
Adams, CL;Nelson, WJ;Smith, SJ
上皮细胞-细胞粘附需要 E-钙粘蛋白相对的细胞外结构域之间以及 E-钙粘蛋白、连环蛋白和肌动蛋白细胞骨架的细胞质结构域之间的相互作用。关于钙粘蛋白-连环蛋白-肌动蛋白复合物如何在细胞与细胞接触时组装，或者这些复合物如何启动和加强粘附，人们知之甚少。我们使用延时微分干涉对比（DIC）成像来观察细胞间接触的发展，并使用定量回顾性免疫细胞化学来测量这些接触中蛋白质的募集。我们表明，E-钙粘蛋白、α-连环蛋白和 β-连环蛋白（但不包括斑珠蛋白）在细胞与细胞接触时共同组装成 Triton X-100 不溶性（TX 不溶性）结构，其动力学类似于增强 E-钙粘蛋白介导的细胞粘附的动力学（Angres, B., A. Barth 和 W.J. Nelson. 1996. J. Cell Biol. 134:549- 557）。 TX不溶性E-钙粘蛋白、α-连环蛋白和β-连环蛋白沿着细胞与细胞接触共定位在空间离散的微域中，我们将其称为“点”，并且每个点中每种蛋白质的相对量成比例增加。随着接触长度的增加，泪点的数量沿着接触点成比例地增加，并且每个泪点都与一束肌动蛋白丝相关联。这些结果表明，E-钙粘蛋白/连环蛋白复合物在斑点中的局部聚集以及它们与肌动蛋白的关联参与了细胞接触的启动。随后，沿着接触的额外泪点的空间排序可能涉及将膜拉在一起，从而导致粘附的快速加强。
Epithelial cell-cell adhesion requires interactions between opposing extracellular domains of E-cadherin, and among the cytoplasmic domain of E-cadherin, catenins, and actin cytoskeleton. Little is known about how the cadherin-catenin-actin complex is assembled upon cell-cell contact, or how these complexes initiate and strengthen adhesion. We have used time-lapse differential interference contrast (DIC) imaging to observe the development of cell-cell contacts, and quantitative retrospective immunocytochemistry to measure recruitment of proteins to those contacts. We show that E-cadherin, alpha-catenin, and beta- catenin, but not plakoglobin, coassemble into Triton X-100 insoluble (TX-insoluble) structures at cell-cell contacts with kinetics similar to those for strengthening of E-cadherin-mediated cell adhesion (Angres, B., A. Barth, and W.J. Nelson. 1996. J. Cell Biol. 134:549- 557). TX-insoluble E-cadherin, alpha-catenin, and beta-catenin colocalize along cell-cell contacts in spatially discrete micro-domains which we designate "puncta," and the relative amounts of each protein in each punctum increase proportionally. As the length of the contact increases, the number of puncta increases proportionally along the contact and each punctum is associated with a bundle of actin filaments. These results indicate that localized clustering of E- cadherin/catenin complexes into puncta and their association with actin is involved in initiating cell contacts. Subsequently, the spatial ordering of additional puncta along the contact may be involved in zippering membranes together, resulting in rapid strengthening of adhesion.
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DOI：
10.1083/jcb.101.4.1307
发表时间：
1985-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Behrens J;Birchmeier W;Goodman SL;Imhof BA
通讯作者：
Imhof BA
影响因子：
7.8
作者：
MCNEILL, H;RYAN, TA;NELSON, WJ
通讯作者：
NELSON, WJ
影响因子：
7.8
作者：
Angres, B;Barth, A;Nelson, WJ
通讯作者：
Nelson, WJ
DOI：
10.1083/jcb.130.5.1105
发表时间：
1995-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mays RW;Siemers KA;Fritz BA;Lowe AW;van Meer G;Nelson WJ
通讯作者：
Nelson WJ
DOI：
10.1083/jcb.107.4.1575
发表时间：
1988-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Gumbiner B;Stevenson B;Grimaldi A
通讯作者：
Grimaldi A