Molecular mapping of the Pi2/9 allelic gene Pi2-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson.
Molecular mapping of the Pi2/9 allelic gene Pi2-2 conferring broad-spectrum resistance to Magnaporthe oryzae in the rice cultivar Jefferson.
复制标题
Pi2/9 等位基因 Pi2-2 的分子图谱赋予水稻品种 Jefferson 对稻瘟病的广谱抗性
DOI：
10.1186/1939-8433-5-29
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发表时间：
2012-12
期刊：
影响因子：
--
通讯作者：
Wang GL
中科院分区：
文献类型：
--
作者：
Jiang N;Li Z;Wu J;Wang Y;Wu L;Wang S;Wang D;Wen T;Liang Y;Sun P;Liu J;Dai L;Wang Z;Wang C;Luo M;Liu X;Wang GL
背景
利用广谱抗病基因是防治稻瘟病菌的一种经济有效的策略。在已克隆的抗稻瘟病基因中，Pi 9、Pi 2和Piz-t对多种稻瘟病具有广谱抗性。从6号染色体上的Pi 2/9基因座分离到了P12/9菌株。从该基因座中分离和鉴定出具有不同抗性谱的抗病基因，将为水稻抗虫育种提供新的遗传资源。
结果
在这项研究中，我们确定了一个显性R基因，Pi 2 -2，在Pi 2/9位点从杰斐逊，一个优秀的美国水稻品种，通过遗传和物理定位。接种试验表明，Jefferson对M. Piz-t分离物比较了具有Pi 9、Pi 2和Piz-t基因的水稻品系。精细定位将Pi 2 -2界定为标记AP 5659 -3和RM 19817之间的270-kb间隔，并且该间隔包含日本晴基因组中的三个核苷酸结合位点-富含亮氨酸重复序列（NBS-LRR）基因。鉴定了5个跨越该区域的细菌人工染色体（BAC）克隆，并构建了覆盖Pi 2 -2位点的BAC重叠群。
结论
我们确定了一个新的等位基因在Pi 2/9位点和精细映射的基因内的270 kb的区域。本研究结果为分离Pi 2 -2基因和紧密连锁的DNA标记用于水稻抗稻瘟病育种提供了重要信息。
Background
Utilization of broad-spectrum resistance (R) genes is an effective and economical strategy to control the fungal pathogen Magnaporthe oryzae, the causal agent of the rice blast disease. Among the cloned blast resistance genes, Pi9, Pi2 and Piz-t confer broad-spectrum resistance to diverse M. oryzae isolates and were isolated from the Pi2/9 locus on chromosome 6. Identification and isolation of additional R genes with different resistance spectra from this locus will provide novel genetic resources for better control of this important rice disease.
Results
In this study, we identified a dominant R gene, Pi2-2, at the Pi2/9 locus from Jefferson, an elite U.S. rice cultivar, through genetic and physical mapping. Inoculation tests showed that Jefferson has different resistant specificities to M. oryzae isolates compared rice lines with the Pi9, Pi2 and Piz-t genes. Fine mapping delimited Pi2-2 to a 270-kb interval between the markers AP5659-3 and RM19817, and this interval contains three nucleotide-binding site-leucine-rich repeat (NBS-LRR) genes in the Nipponbare genome. Five bacterial artificial chromosome (BAC) clones spanning the region were identified, and a BAC contig covering the Pi2-2 locus was constructed.
Conclusions
We identified a new allelic gene at the Pi2/9 locus and fine-mapped the gene within a 270-kb region. Our results provide essential information for the isolation of the Pi2-2 gene and tightly linked DNA markers for rice blast resistance breeding.
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影响因子：
14.9
作者：
Ouyang S;Zhu W;Hamilton J;Lin H;Campbell M;Childs K;Thibaud-Nissen F;Malek RL;Lee Y;Zheng L;Orvis J;Haas B;Wortman J;Buell CR
通讯作者：
Buell CR
影响因子：
5.4
作者：
Hayashi, K;Hashimoto, N;Ashikawa, I
通讯作者：
Ashikawa, I
影响因子：
3.3
作者：
Liu, Xinqiong;Lin, Fei;Pan, Qinghua
通讯作者：
Pan, Qinghua
影响因子：
5.4
作者：
Conaway-Bormans, CA;Marchetti, MA;Park, WD
通讯作者：
Park, WD
影响因子：
2.3
作者：
McClung, AM;Marchetti, MA;Bollich, CN
通讯作者：
Bollich, CN