Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes.
Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes.
复制标题
DOI：
10.1371/journal.pone.0028367
复制
发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kanai Y
中科院分区：
文献类型：
--
作者：
Sato T;Aiyama Y;Ishii-Inagaki M;Hara K;Tsunekawa N;Harikae K;Uemura-Kamata M;Shinomura M;Zhu XB;Maeda S;Kuwahara-Otani S;Kudo A;Kawakami H;Kanai-Azuma M;Fujiwara M;Miyamae Y;Yoshida S;Seki M;Kurohmaru M;Kanai Y
胶质细胞源性神经营养因子（glial cell line-derived neurotrophic factor，GDNF）是哺乳动物精子发生过程中主要的支持细胞源性因子之一，通过GDNF家族受体α1（glial cell line-derived neurotrophic factor，GFRα1）调控未分化精原细胞包括精原干细胞（spermatogonial stem cells，SSC）的维持。GDNF分子在体内何时、何地以及如何产生并暴露于GFRα1阳性精原细胞中仍不清楚。在此，我们显示了免疫反应性GDNF阳性信号的周期性和斑块样分布，以及它们与GFRα1阳性精原细胞沿着支持细胞基底面的一个亚群的密切共定位。抗GDNF切片免疫组化显示GDNF阳性信号主要在细胞质中，并以种属特异性以及生精周期和生精活性依赖性的方式在支持细胞中特异性地观察到。与小鼠睾丸中普遍存在的GDNF信号相反，在精子形成之前在仓鼠睾丸中周期性地观察到高水平的GDNF信号。整体安装抗GDNF染色的曲细精管成功地可视化GDNF阳性颗粒沉积物沿着在两个物种的支持细胞的基底表面的周期性和补丁样的细胞外分布。GDNF和GFRα1的双染显示GDNF沉积和GFRα1阳性精原细胞亚群的密切共定位。在这两个物种中，GFRα1阳性细胞显示出细长的双极形状，以及在GDNF富集区的细胞数量增加的趋势，与在曲细精管的GDNF低/阴性区的细胞相比。我们的数据提供了直接的证据，区域定义的补丁样GDNF阳性信号位点，GFRα1阳性精原细胞可能与GDNF相互作用的曲细精管基底室。
In mammalian spermatogenesis, glial cell line-derived neurotrophic factor (GDNF) is one of the major Sertoli cell-derived factors which regulates the maintenance of undifferentiated spermatogonia including spermatogonial stem cells (SSCs) through GDNF family receptor α1 (GFRα1). It remains unclear as to when, where and how GDNF molecules are produced and exposed to the GFRα1-positive spermatogonia in vivo. Here we show the cyclical and patch-like distribution of immunoreactive GDNF-positive signals and their close co-localization with a subpopulation of GFRα1-positive spermatogonia along the basal surface of Sertoli cells in mice and hamsters. Anti-GDNF section immunostaining revealed that GDNF-positive signals are mainly cytoplasmic and observed specifically in the Sertoli cells in a species-specific as well as a seminiferous cycle- and spermatogenic activity-dependent manner. In contrast to the ubiquitous GDNF signals in mouse testes, high levels of its signals were cyclically observed in hamster testes prior to spermiation. Whole-mount anti-GDNF staining of the seminiferous tubules successfully visualized the cyclical and patch-like extracellular distribution of GDNF-positive granular deposits along the basal surface of Sertoli cells in both species. Double-staining of GDNF and GFRα1 demonstrated the close co-localization of GDNF deposits and a subpopulation of GFRα1-positive spermatogonia. In both species, GFRα1-positive cells showed a slender bipolar shape as well as a tendency for increased cell numbers in the GDNF-enriched area, as compared with those in the GDNF-low/negative area of the seminiferous tubules. Our data provide direct evidence of regionally defined patch-like GDNF-positive signal site in which GFRα1-positive spermatogonia possibly interact with GDNF in the basal compartment of the seminiferous tubules.
登录
查看更多内容
影响因子：
64.5
作者：
Hobbs RM;Seandel M;Falciatori I;Rafii S;Pandolfi PP
通讯作者：
Pandolfi PP
影响因子：
4.1
作者：
Hofmann, Marie-Claude
通讯作者：
Hofmann, Marie-Claude
影响因子：
2.8
作者：
Aponte, PM;de Rooij, DG;Bastidas, P
通讯作者：
Bastidas, P
影响因子：
2.7
作者：
Hofmann, MC;Braydich-Stolle, L;Dym, M
通讯作者：
Dym, M
影响因子：
3.6
作者：
Kanatsu-Shinohara, Mito;Muneto, Tomomi;Shinohara, Takashi
通讯作者：
Shinohara, Takashi