Lectin-Seq: A method to profile lectin-microbe interactions in native communities.
Lectin-Seq: A method to profile lectin-microbe interactions in native communities.
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DOI：
10.1126/sciadv.add8766
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发表时间：
2023-07-28
期刊：
影响因子：
13.6
通讯作者：
中科院分区：
文献类型：
--
作者：
可溶性人凝集素是先天免疫的重要组成部分。遗传模型表明，凝集素影响宿主微生物群，但它们对共生和共生物种的特异性尚未得到充分研究。阐明凝集素在调节微生物群中的作用需要了解它们在本地群落中结合的微生物物种。为了分析人类凝集素识别，我们开发了凝集素- seq。我们使用可溶性甘露糖结合凝集素（MBL）和智力素-1 （hItln1）将凝集素- seq应用于人类粪便微生物群。尽管每种凝集素都能结合相当比例的样品（10 - 20%），但MBL和hItln1的微生物相互作用组在组成和多样性上存在显著差异。MBL结合对通常与人类相关的一小部分物种具有高度选择性。相比之下，hItln1的相互作用谱包含了广泛的低丰度物种。我们的数据揭示了人类凝集素共栖识别特性的明显差异。可溶性人凝集素结合粪便微生物群中不同的细菌种类。
Soluble human lectins are critical components of innate immunity. Genetic models suggest that lectins influence host-resident microbiota, but their specificity for commensal and mutualist species is understudied. Elucidating lectins’ roles in regulating microbiota requires an understanding of which microbial species they bind within native communities. To profile human lectin recognition, we developed Lectin-Seq. We apply Lectin-Seq to human fecal microbiota using the soluble mannose-binding lectin (MBL) and intelectin-1 (hItln1). Although each lectin binds a substantial percentage of the samples (10 to 20%), the microbial interactomes of MBL and hItln1 differ markedly in composition and diversity. MBL binding is highly selective for a small subset of species commonly associated with humans. In contrast, hItln1’s interaction profile encompasses a broad range of lower-abundance species. Our data uncover stark differences in the commensal recognition properties of human lectins. Soluble human lectins bind distinct bacterial species in fecal microbiota.
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影响因子：
9.2
作者：
Lugli, Gabriele Andrea;Mancabelli, Leonardo;Milani, Christian;Fontana, Federico;Tarracchini, Chiara;Alessandri, Giulia;van Sinderen, Douwe;Turroni, Francesca;Ventura, Marco
通讯作者：
Ventura, Marco
影响因子：
5.7
作者：
通讯作者：
--
影响因子：
2
作者：
Kobayashi, Hidetoshi;Uchimura, Kohei;Furuya, Fumihiko
通讯作者：
Furuya, Fumihiko
影响因子：
15.5
作者：
Jackson MA;Pearson C;Ilott NE;Huus KE;Hegazy AN;Webber J;Finlay BB;Macpherson AJ;Powrie F;Lam LH
通讯作者：
Lam LH
影响因子：
14.9
作者：
Drula E;Garron ML;Dogan S;Lombard V;Henrissat B;Terrapon N
通讯作者：
Terrapon N