Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats.
Egg ovotransferrin-derived ACE inhibitory peptide IRW increases ACE2 but decreases proinflammatory genes expression in mesenteric artery of spontaneously hypertensive rats.
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DOI：
10.1002/mnfr.201500050
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发表时间：
2015-09
影响因子：
5.2
通讯作者：
Wu, Jianping
中科院分区：
文献类型：
--
作者：
Majumder, Kaustav;Liang, Guanxiang;Chen, Yanhong;Guan, LeLuo;Davidge, Sandra T.;Wu, Jianping
鸡蛋转铁蛋白衍生的血管紧张素转换酶（ACE）抑制肽IRW先前被证明可以通过减少血管炎症和增加一氧化氮介导的血管舒张来降低自发性高血压大鼠的血压。本研究的主要目的是通过RNAseq技术对该肽进行转录组分析，探讨其分子机制。从肾和肠系膜动脉提取总RNA;构建RNAseq文库（来自未处理组和IRW处理组），并使用HiSeq 2000系统（Illumina）进行测序。在肾动脉和肠系膜动脉中分别检测到12764和13352个基因。鉴定了未处理组和IRW处理组之间的差异表达（DE）基因，通过独创性途径分析进行的功能分析显示，从肠系膜动脉中鉴定的DE基因在调节各种心血管功能方面的作用大于肾脏。随后的qPCR分析进一步证实，IRW显著增加肠系膜动脉中ACE-2、ABCB-1、IRF-8和CDH-1的表达，同时显著降低ICAM-1和VCAM-1的表达。我们的研究首次表明，ACE抑制肽IRW可能通过增加ACE 2和降低促炎基因表达而发挥其降压活性。
Egg ovotransferrin‐derived angiotensin converting enzyme (ACE) inhibitory peptide IRW was previously shown to reduce blood pressure in spontaneously hypertensive rats through reduced vascular inflammation and increased nitric oxide‐mediated vasorelaxation. The main objective of the present study was to investigate the molecular mechanism of this peptide through transcriptome analysis by RNAseq technique. Total RNA was extracted from kidney and mesenteric arteries; the RNAseq libraries (from untreated and IRW‐treated groups) were constructed and subjected to sequence using HiSeq 2000 system (Illumina) system. A total of 12 764 and 13 352 genes were detected in kidney and mesenteric arteries, respectively. The differentially expressed (DE) genes between untreated and IRW‐treated groups were identified and the functional analysis through ingenuity pathway analysis revealed a greater role of DE genes identified from mesenteric arteries than that of kidney in modulating various cardiovascular functions. Subsequent qPCR analysis further confirmed that IRW significantly increased the expression of ACE‐2, ABCB‐1, IRF‐8, and CDH‐1 while significantly decreased the expression ICAM‐1 and VCAM‐1 in mesenteric arteries. Our research showed for the first time that ACE inhibitory peptide IRW could contribute to its antihypertensive activity through increased ACE2 and decreased proinflammatory genes expression.
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影响因子：
3.6
作者：
Aihara, Kotaro;Osaka, Mizuko;Yoshida, Masayuki
通讯作者：
Yoshida, Masayuki
影响因子：
8.8
作者：
Bansode, Rishipal R.;Randolph, Priscilla;Ahmedna, Mohamed
通讯作者：
Ahmedna, Mohamed
影响因子：
2.7
作者：
Cicero, A. F. G.;Gerocarni, B.;Borghi, C.
通讯作者：
Borghi, C.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
6.2
作者：
Chockalingam, Arun
通讯作者：
Chockalingam, Arun