Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne bla(OXA-72) in Taiwan.
Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne bla(OXA-72) in Taiwan.
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DOI：
10.1186/1471-2334-13-319
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发表时间：
2013-07-13
影响因子：
3.7
通讯作者：
Fung CP
中科院分区：
文献类型：
--
作者：
Kuo SC;Yang SP;Lee YT;Chuang HC;Chen CP;Chang CL;Chen TL;Lu PL;Hsueh PR;Fung CP
台湾多中心对亚胺培南耐药鲍曼不动杆菌 (IRAB) 的系统监测显示，出现了 blaOXA-72 菌株。这项研究描述了它们的基因组成、传播机制以及对碳青霉烯类耐药性的贡献。 2007年6月至2007年9月，从台湾不同地理区域的10家教学医院收集了291株鲍曼不动杆菌非重复分离株。通过琼脂稀释法测定最低抑菌浓度（MIC）。通过脉冲场凝胶电泳测定克隆性。提取质粒并用限制性酶消化，随后通过电泳和Southern印迹分析blaOXA-72。通过反向PCR确定blaOXA-72的侧翼区域。 blaOXA-72 对亚胺培南 MIC 的贡献通过将携带 blaOXA-72 的质粒转化到亚胺培南敏感鲍曼不动杆菌中来确定。台湾142个IRAB中，27个携带blaOXA-72；南台湾22人，中台湾5人，北台湾无一人。有两个主要的克隆。在所有分离株的质粒中均鉴定出 blaOXA-72。鉴定出质粒携带的 blaOXA-72 侧翼的两个遗传结构，并且在某些区域具有相同的序列；先前文献中描述的一种仅存在于一种分离株中，而新的一种则存在于其余分离株中。 blaOXA-72 的引入导致转化体中亚胺培南 MIC 的增加。 blaOXA-72 mRNA 对亚胺培南的过度表达进一步支持了 blaOXA-72 的贡献。总之，携带新质粒的 blaOXA-72 分离株已在台湾南部成功传播。抗性基因的传播取决于新质粒的克隆传播和传播。这些分离株中的 BlaOXA-72 直接导致其对亚胺培南产生耐药性。
The systemic surveillance of imipenem-resistant Acinetobacter baumannii (IRAB) from multicenters in Taiwan revealed the emergence of isolates with blaOXA-72. This study described their genetic makeup, mechanism of spread, and contribution to carbapenem resistance. Two hundred and ninety-one non-repetitive isolates of A. baumannii were collected from 10 teaching hospitals from different geographical regions in Taiwan from June 2007 to September 2007. Minimal inhibitory concentrations (MICs) were determined by agar dilution. Clonality was determined by pulsed-field gel electrophoresis. Plasmid was extracted and digested by restriction enzymes, and subsequently analyzed by electrophoresis and Southern blot for blaOXA-72. The flanking regions of blaOXA-72 were determined by inverse PCR. The contribution of blaOXA-72 to imipenem MIC was determined by transforming plasmids carrying blaOXA-72 into imipenem-susceptible A. baumannii. Among 142 IRAB in Taiwan, 27 harbored blaOXA-72; 22 originated from Southern Taiwan, 5 from Central Taiwan, and none from Northern Taiwan. There were two major clones. The blaOXA-72 was identified in the plasmids of all isolates. Two genetic structures flanking plasmid-borne blaOXA-72 were identified and shared identical sequences in certain regions; the one described in previous literature was present in only one isolate, and the new one was present in the remaining isolates. Introduction of blaOXA-72 resulted in an increase of imipenem MIC in the transformants. The overexpression of blaOXA-72 mRNA in response to imipenem further supported the contribution of blaOXA-72. In conclusion, isolates with new plasmid-borne blaOXA-72 were found to be disseminated successfully in Southern Taiwan. The spread of the resistance gene depended on clonal spread and dissemination of a new plasmid. BlaOXA-72 in these isolates directly led to their imipenem-resistance.
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