A Synthetic Peptide Corresponding to the Rab4 Hypervariable Carboxyl-terminal Domain Inhibits Insulin Action on Glucose Transport in Rat Adipocytes (*)
A Synthetic Peptide Corresponding to the Rab4 Hypervariable Carboxyl-terminal Domain Inhibits Insulin Action on Glucose Transport in Rat Adipocytes (*)
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与 Rab4 高变羧基末端结构域相对应的合成肽抑制胰岛素对大鼠脂肪细胞葡萄糖转运的作用 (*)
DOI：
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发表时间：
1996
影响因子：
4.8
通讯作者：
I. Kojima
中科院分区：
文献类型：
--
作者：
H. Shibata;W. Omata;Yoichi Suzuki;Shigeyasu Tanaka;I. Kojima
The present study was conducted to examine the involvement of Rab4, a low molecular weight GTP-binding protein, in the action of insulin on glucose transport. A synthetic peptide corresponding to the Rab4 hypervariable carboxyl-terminal domain, Rab4-(191-210), was successfully transferred into rat adipocytes by electroporation and inhibited insulin-stimulated glucose transport by about 50% without affecting the basal transport activity. In contrast, synthetic peptides corresponding to the Rab3C and Rab3D carboxyl-terminal hypervariable domain had little effect on insulin action on glucose transport. The Rab4-(191-210) peptide also reduced insulin-induced GLUT4 translocation from the intracellular pool to the plasma membrane. Furthermore, the Rab4-(191-210) peptide reduced both insulin-induced glucose transport and GLUT4 translocation in the presence of a major histocompatibility complex class I antigen-derived peptide, D-(62-85), which is a potent inhibitor of GLUT4 internalization, suggesting that the peptide inhibited exocytotic recruitment of GLUT4-containing vesicles. The Rab4-(191-210) peptide also inhibited GTPS-stimulated glucose transport. In addition, insulin-stimulated glucose transport was inhibited by the addition of anti-Rab4 antibody. These results suggest that Rab4 protein plays a crucial role in insulin action on GLUT4 translocation, especially in exocytotic recruitment by the hormone of the glucose transporter to the plasma membrane from the intracellular retention pool.
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在α-毒素透化的大鼠脂肪细胞中，胰岛素和不可水解的 GTP 类似物诱导 GLUT 4 易位至质膜。
DOI：
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发表时间：
1991
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Baldini,G;Hohman,R;Charron,MJ;Lodish,HF
通讯作者：
Lodish,HF
DOI：
10.1073/pnas.89.11.5049
发表时间：
1992-06-01
影响因子：
11.1
作者：
BALDINI, G;HOHL, T;LODISH, HF
通讯作者：
LODISH, HF
DOI：
--
发表时间：
1986-07
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
R. Davis;S. Corvera;M. Czech
通讯作者：
R. Davis;S. Corvera;M. Czech
蛋白激酶 C 可能不参与胰岛素对 cAMP 磷酸二酯酶作用的证据：对对胰岛素高度敏感的电穿孔大鼠脂肪细胞进行的研究。
影响因子：
3.9
作者：
Shibata,H;Robinson,FW;Benzing,CF;Kono,T
通讯作者：
Kono,T
DOI：
10.1016/s0021-9258(19)49748-2
发表时间：
1992-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Cynthia A. Corley;Cain;William S Trimblez;Gustav E Lienhardq
通讯作者：
Cynthia A. Corley;Cain;William S Trimblez;Gustav E Lienhardq