Region-dependent bone loss in the lumbar spine following femoral fracture in mice.
Region-dependent bone loss in the lumbar spine following femoral fracture in mice.
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DOI：
10.1016/j.bone.2020.115555
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发表时间：
2020-11
期刊：
影响因子：
4.1
通讯作者：
Christiansen BA
中科院分区：
文献类型：
--
作者：
Ely EV;Osipov B;Emami AJ;Christiansen BA
我们之前的研究表明，股骨骨折后，小鼠远端骨骼部位（包括腰椎）的骨丢失。这种骨丢失可能增加随后椎体骨折的风险，特别是在高应变骨区域（最常发生在椎体上、下终板附近）发生骨丢失。为了确定股骨骨折后腰椎的局部骨质流失，我们评估了年轻（3个月）和中年（12个月）雌性C57BL/6小鼠在股骨横向骨折两周后L5椎体的颅骨、中心和尾端部分，并与年轻和中年未受伤的对照小鼠进行了比较。我们假设，在年轻和中年小鼠中，颅骨和尾侧区域的骨质流失比中央区更大。采用显微计算机断层扫描评估骨小梁和骨皮质的微观结构，并对破骨细胞数量和侵蚀面进行组织学评估。在幼龄小鼠中，骨折主要导致颅骨和尾侧区域骨小梁和皮质骨微结构的下降，而在中心区没有，而在中年小鼠中，骨折后所有区域的骨小梁都减少，但皮质骨微结构没有任何变化。在此时间点，破骨细胞数量和侵蚀面无明显差异。这些数据表明，年轻小鼠骨折后的骨质流失主要集中在含有大量高应变组织的区域，而中年小鼠的骨质流失则较少依赖于区域，仅限于骨小梁间室。这些结果说明了骨折后系统性骨质流失如何导致椎体强度下降，以及不同区域模式和年龄依赖性骨质流失差异如何不同地影响椎体骨折风险。
We previously showed that after femur fracture, mice lose bone at distant skeletal sites, including the lumbar vertebrae. This bone loss may increase the risk of subsequent vertebral fractures, particularly if bone is lost from high-strain bone regions, which are most commonly found adjacent to the superior and inferior endplates of the vertebral body. To determine regional bone loss from the lumbar spine following femur fracture, we evaluated the cranial, center, and caudal portions of the L5 vertebral bodies of Young (3 month-old) and Middle-Aged (12 month-old) female C57BL/6 mice two weeks after a transverse femur fractures compared to Young and Middle-Aged uninjured control mice. We hypothesized that greater bone loss would be observed in the cranial and caudal regions than in the center region in both Young and Middle-Aged mice. Trabecular and cortical bone microstructure were evaluated using micro-computed tomography, and osteoclast number and eroded surface were evaluated histologically. In Young Mice, fracture led to decreased trabecular and cortical bone microstructure primarily in the cranial and caudal regions, but not the center region, while Middle-Aged mice demonstrated decreases in trabecular bone in all regions, but did not exhibit any changes in cortical bone microstructure after fracture. No significant differences in osteoclast number or eroded surface were observed at this time point. These data suggest that bone loss following fracture in Young Mice is concentrated in areas that contain a large amount of high-strain tissue, whereas bone loss in Middle-Aged mice is less region-dependent and is restricted to the trabecular bone compartment. These results illustrate how systemic bone loss after fracture could lead to decreases in vertebral strength, and how distinct regional patterns and age-dependent differences in bone loss may differentially affect vertebral fracture risk.
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3
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