Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.
Mass Spectrometry Approaches to Glycomic and Glycoproteomic Analyses.
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DOI：
10.1021/acs.chemrev.7b00732
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发表时间：
2018-09-12
期刊：
影响因子：
62.1
通讯作者：
Lebrilla CB
中科院分区：
文献类型：
--
作者：
Ruhaak LR;Xu G;Li Q;Goonatilleke E;Lebrilla CB
糖组学和糖蛋白质组学分析涉及对与蛋白质结合的寡糖（聚糖）的特性研究。聚糖是通过一个复杂的非模板驱动过程产生的，该过程涉及延伸新生链的酶之间的竞争。结构的高度多样性、单个糖残基极性的差异以及聚糖较差的电离效率，都使得聚糖分析比任何其他生物聚合物的分析都要困难得多。此外，与特定蛋白质位点相关的大量糖型使得其比任何翻译后修饰都更难以表征。尽管如此，已经取得了重大进展，先进的分离和质谱方法处于核心地位，也是取得进展的主要原因。虽然糖组学和糖蛋白质组学分析通常仍然只能在高度专业化的实验室中进行，但新的软件和工作流程使其更容易获得。本综述重点关注质谱和分离方法在推动糖组学和糖蛋白质组学分析方面的作用。它描述了该领域的现状以及为使更广泛的科学界更容易获得相关技术所取得的进展。
Glycomic and glycoproteomic analyses involve the characterization of oligosaccharides (glycans) conjugated to proteins. Glycans are produced through a complicated nontemplate driven process involving the competition of enzymes that extend the nascent chain. The large diversity of structures, the variations in polarity of the individual saccharide residues, and the poor ionization efficiencies of glycans all conspire to make the analysis arguably much more difficult than any other biopolymer. Furthermore, the large number of glycoforms associated with a specific protein site makes it more difficult to characterize than any post-translational modification. Nonetheless, there have been significant progress, and advanced separation and mass spectrometry methods have been at its center and the main reason for the progress. While glycomic and glycoproteomic analyses are still typically available only through highly specialized laboratories, new software and workflow is making it more accessible. This review focuses on the role of mass spectrometry and separation methods in advancing glycomic and glycoproteomic analyses. It describes the current state of the field and progress toward making it more available to the larger scientific community.
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影响因子：
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作者：
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