The mouse X chromosome is enriched for multicopy testis genes showing postmeiotic expression.
The mouse X chromosome is enriched for multicopy testis genes showing postmeiotic expression.
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DOI：
10.1038/ng.126
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发表时间：
2008-06
期刊：
影响因子：
30.8
通讯作者：
Turner, James M. A.
中科院分区：
文献类型：
--
作者：
Mueller, Jacob L.;Mahadevaiah, Shantha K.;Park, Peter J.;Warburton, Peter E.;Page, David C.;Turner, James M. A.
目前普遍认为，哺乳动物X染色体在减数分裂前表达的生精基因丰富，减数分裂后表达的生精基因缺乏。X染色体上减数分裂后基因的缺乏被解释为减数分裂性染色体失活（MSCI）的结果-在减数分裂前期XY二价基因的完全沉默。最近的研究得出结论，msci引发的沉默持续到减数分裂之后，大多数x基因在圆形精子中仍然被抑制。我们在此报告了33个多拷贝基因家族，代表约273个小鼠x连锁基因，在睾丸中表达，并且这种表达主要在减数分裂后细胞中表达。RNA FISH和微阵列分析表明，X染色体减数分裂后抑制的维持是不完整的。此外，x连锁的多拷贝基因表现出与常染色体基因相似的表达水平。因此，小鼠X染色体不仅富含减数分裂前功能的精子发生基因，而且约18%的小鼠X连锁基因表现出减数分裂后的表达。
According to the prevailing view, mammalian X chromosomes are enriched in spermatogenesis genes expressed before meiosis and deficient in spermatogenesis genes expressed after meiosis. The paucity of post-meiotic genes on the X chromosome has been interpreted as a consequence of Meiotic Sex Chromosome Inactivation (MSCI) – the complete silencing of genes on the XY bivalent at meiotic prophase. Recent studies have concluded that MSCI-initiated silencing persists beyond meiosis and that most X-genes remain repressed in round spermatids. We report here that 33 multi-copy gene families, representing ~273 mouse X-linked genes, are expressed in the testis and that this expression is predominantly in post-meiotic cells. RNA FISH and microarray analysis show that the maintenance of X chromosome post-meiotic repression is incomplete. Furthermore, X-linked multi-copy genes exhibit expression levels similar to those of autosomal genes. Thus, not only is the mouse X chromosome enriched for spermatogenesis genes functioning before meiosis, but in addition ~18% of mouse X-linked genes exhibit post-meiotic expression.
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