Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2alpha-induced regression in sheep.
Vascular composition, apoptosis, and expression of angiogenic factors in the corpus luteum during prostaglandin F2alpha-induced regression in sheep.
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前列腺素 F2α 诱导绵羊退化过程中黄体中的血管组成、细胞凋亡和血管生成因子的表达。
DOI：
10.1530/rep.1.01062
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发表时间：
2006
期刊：
影响因子：
3.8
通讯作者：
A. Grazul
中科院分区：
文献类型：
--
作者：
K. Vonnahme;D. Redmer;E. Borowczyk;J. Bilski;J. Luther;M. L. Johnson;L. Reynolds;A. Grazul
在发情周期第10天，注射前列腺素F（2 α）（PGF）类似物后0、4、8、12和24 h，从超排母羊中采集黄体和血液样品。测定了黄体（CL）中血管细胞和成纤维细胞组成、细胞凋亡和几种血管生成因子mRNA表达的变化。注射PGF后24 h外周血孕酮浓度下降，CL重量无变化。前列腺素F处理后，黄体组织中BS-1凝集素染色（内皮细胞标记）、平滑肌细胞肌动蛋白（SMCA;周细胞和SMC标记）、1型胶原（成纤维细胞标记）阳性面积和细胞死亡率发生变化。与这些细胞变化相关的是，包括血管内皮生长因子（VEGF）和受体（Flt和KDR）、碱性成纤维细胞生长因子（FGF 2）和受体、血管生成素（ANGPT）1和受体Tie-2、内皮一氧化氮合酶（NOS 3）和血管紧张素II受体1（AT 1）在内的几种血管生成因子的mRNA发生了改变。内皮细胞标志物表达的变化与VEGF和NO系统的变化呈正相关。此外，VEGF、Flt和KDR mRNA表达的变化与ANGPT 2、Tie-2和NOS 3的变化呈正相关，表明存在功能关系。这些数据表明，在最初的增加后，血管床的内皮成分减少，在前列腺素F诱导的黄体退化。然而，SMCA表达在黄体退化期间保持高水平，可能表明周细胞和血管SMC在黄体溶解中的作用，可能调节组织重塑并维持较大血管的完整性。此外，似乎早期退化可增加成纤维细胞的1型胶原蛋白产生和/或表达。血管生成因子的表达受前列腺素F诱导的黄体溶解的影响，并可能有助于维持血管结构，以帮助黄体退化。
Corpora lutea and blood samples were collected from superovulated ewes 0, 4, 8, 12 and 24 h after prostaglandin F(2alpha) (PGF) analog injection on day 10 of the estrous cycle. Changes in vascular cell and fibroblast composition, apoptosis and mRNA expression for several angiogenic factors in the corpus luteum (CL) were determined. While peripheral progesterone concentration decreased at 24 h after PGF injection, CL weight did not change. The area of positive BS-1 lectin staining (endothelial cell marker), smooth muscle cell actin (SMCA; pericyte and SMC marker), collagen type 1 (fibroblast marker), and the rate of cell death changed in luteal tissues after PGF treatment. In association with these cellular changes, mRNA for several angiogenic factors including vascular endothelial growth factor (VEGF) and receptors (Flt and KDR), basic fibroblast growth factor (FGF2) and receptor, angiopoietin (ANGPT) 1 and receptor Tie-2, endothelial nitric oxide synthase (NOS3), and angiotensin II receptor 1 (AT1) were altered. Changes in endothelial cell marker expression were positively correlated with changes in VEGF and NO systems. In addition, changes in mRNA expression for VEGF, Flt and KDR were positively correlated with changes in ANGPT2, Tie-2, and NOS3, indicating a functional relationship. This data demonstrates that after an initial increase, the endothelial component of the vascular bed decreases during PGF-induced luteal regression. However, SMCA expression remained high during luteal regression, potentially indicating a role of pericytes and vascular SMC in luteolysis, likely to regulate tissue remodeling and to maintain the integrity of larger blood vessels. Further, it appears that early regression may increase collagen type 1 production and/or expression by fibroblasts. Expression of angiogenic factors is influenced by PGF-induced luteolysis and may serve to maintain vascular structure in order to aid luteal regression.
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影响因子：
3.6
作者：
F. Shi;R. Stewart;E. Perez;J. Chen;P. Lapolt
通讯作者：
F. Shi;R. Stewart;E. Perez;J. Chen;P. Lapolt
影响因子：
3.6
作者：
FARIN, CE;MOELLER, CL;NISWENDER, GD
通讯作者：
NISWENDER, GD
影响因子：
3.3
作者：
Silvia,WJ;Niswender,GD
通讯作者：
Niswender,GD
DOI：
10.1073/pnas.86.12.4544
发表时间：
1989-06-01
影响因子：
11.1
作者：
ANTONELLIORLIDGE, A;SAUNDERS, KB;DAMORE, PA
通讯作者：
DAMORE, PA