Limiting Injury During Saphenous Vein Graft Preparation For Coronary Arterial Bypass Prevents Metabolic Decompensation.
Limiting Injury During Saphenous Vein Graft Preparation For Coronary Arterial Bypass Prevents Metabolic Decompensation.
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DOI：
10.1038/s41598-017-13819-w
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发表时间：
2017-10-27
影响因子：
4.6
通讯作者：
Flynn CR
中科院分区：
文献类型：
--
作者：
Cheung-Flynn J;Song J;Voskresensky I;Wise ES;Liu Y;Xiong Y;Eagle SS;Brophy CM;Flynn CR
自体冠状动脉和外周动脉旁路手术标准采集和制备人隐静脉（HSV）与损伤和氧化应激增加有关，这对移植物的性能产生负面影响。在这项研究中，我们研究了HSV在标准静脉移植制备（AP）之前（未准备；UP）和之后的全球代谢组学特征。AP-HSV表现出血管舒缩功能受损，这与氧化应激、磷脂水解和能量消耗增加有关，这是机械和化学损伤的特征。利用猪模型（PSV）验证HSV的这些代谢组学变化，并确定一种改进的制备技术（OP）的有效性，该技术使用压力调节膨胀、无毒静脉标记物和移植物储存在缓冲PlasmaLyte溶液中，以限制移植物制备引起的代谢失代偿。在AP-PSV中观察到的血管舒缩功能和代谢特征的缺陷可以通过OP手术在很大程度上减轻。这些发现表明，旨在减少移植物收获和准备过程中的损伤的简单策略是一种直接可行的策略，可以保护导管功能并可能改善移植物通畅。
Standard harvest and preparation of human saphenous vein (HSV) for autologous coronary and peripheral arterial bypass procedures is associated with injury and increased oxidative stress that negatively affect graft performance. In this study we investigated the global metabolomic profiles of HSV before (unprepared; UP) and after standard vein graft preparation (AP). AP-HSV showed impaired vasomotor function that was associated with increased oxidative stress, phospholipid hydrolysis and energy depletion that are characteristic of mechanical and chemical injury. A porcine model (PSV) was utilized to validate these metabolomic changes in HSV and to determine the efficacy of an improved preparation technique (OP) using pressure-regulated distension, a non-toxic vein marker, and graft storage in buffered PlasmaLyte solution in limiting metabolic decompensation due to graft preparation. Deficits in vasomotor function and metabolic signature observed in AP-PSV could be largely mitigated with the OP procedure. These findings suggest that simple strategies aimed at reducing injury during graft harvest and preparation represents a straightforward and viable strategy to preserve conduit function and possibly improve graft patency.
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影响因子：
16.9
作者：
Harskamp, Ralf E.;Alexander, John H.;Schulte, Phillip J.;Brophy, Colleen M.;Mack, Michael J.;Peterson, Eric D.;Williams, Judson B.;Gibson, C. Michael;Califf, Robert M.;Kouchoukos, Nicholas T.;Harrington, Robert A.;Ferguson, T. Bruce, Jr.;Lopes, Renato D.
通讯作者：
Lopes, Renato D.
影响因子：
4.8
作者：
CAMACHO, M;GODESSART, N;VILA, L
通讯作者：
VILA, L
影响因子：
6
作者：
Greig, Fiona H.;Hutchison, Lisa;Kennedy, Simon
通讯作者：
Kennedy, Simon
影响因子：
4.3
作者：
Byrom, Michael J.;Bannon, Paul G.;Ng, Martin K. C.
通讯作者：
Ng, Martin K. C.
影响因子：
8.6
作者：
Dehaven CD;Evans AM;Dai H;Lawton KA
通讯作者：
Lawton KA