Increased sialylation of anti-Thomsen-Friedenreich antigen (CD176) antibodies in patients with gastric cancer: a diagnostic and prognostic potential.
Increased sialylation of anti-Thomsen-Friedenreich antigen (CD176) antibodies in patients with gastric cancer: a diagnostic and prognostic potential.
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DOI：
10.1155/2014/830847
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发表时间：
2014
影响因子：
--
通讯作者：
Sergeyev B
中科院分区：
文献类型：
--
作者：
Kurtenkov O;Izotova J;Klaamas K;Sergeyev B
瞄准目的：研究特异性抗TF糖表位抗体（Ab）唾液酸化的改变是否对胃癌的诊断和预后具有潜在意义。方法.血清样本取自胃癌患者（n = 142）和对照组（n = 61）。以合成的TF-聚丙烯酰胺偶联物为抗原，以唾液酸特异性接骨木凝集素（SNA）为抗原，采用ELISA法检测TF特异性抗体及其唾液酸化水平。结果恶性肿瘤患者血清TF特异性IgM水平显著低于对照组（P ≤ 0.001）。肿瘤患者SNA与抗TF IgM和伊加的结合水平较高（P ≤ 0.001），与疾病分期、肿瘤形态和性别无关。SNA/Ab指数的变化对胃癌的敏感性（66-71%）和特异性（60-73%）中等。SNA结合率高、抗TF IgM水平低的患者诊断准确率最高（100%），占29%。这部分患者的生存率最低。结论我们的研究结果首次证明TF特异性抗体的唾液酸化增加与低水平的抗TF IgM结合与胃癌和患者生存密切相关，可用作癌症检测和预后的新生物标志物。
Aim. To study whether alterations in the sialylation of antibodies (Ab) specific to the Thomsen-Friedenreich (TF) glycotope have a diagnostic and prognostic potential in gastric cancer. Methods. Serum samples were taken from patients with gastric carcinoma (n = 142) and controls (n = 61). The level of TF-specific antibodies and their sialylation was detected using ELISA with synthetic TF-polyacrylamide conjugate as antigen and sialic acid-specific Sambucus nigra agglutinin (SNA). Results. The level of TF-specific IgM was significantly decreased in cancer compared with controls (P ≤ 0.001). Cancer patients showed a higher level of SNA binding to anti-TF IgM and IgA (P ≤ 0.001) irrespective of disease stage, tumor morphology, and gender. Changes in the SNA/Ab index demonstrated moderate sensitivity (66–71%) and specificity (60–73%) for stomach cancer. The best diagnostic accuracy (100%) was achieved in 29% patients with high SNA binding and low anti-TF IgM level. This subset of patients demonstrated the poorest survival. Conclusion. Our findings are the first evidence that the increased sialylation of TF-specific Abs combined with a low level of anti-TF IgM is strongly linked to gastric cancer and patients survival, which can be used as a novel biomarker for cancer detection and prognosis.
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影响因子：
3.8
作者：
Iida S;Kuni-Kamochi R;Mori K;Misaka H;Inoue M;Okazaki A;Shitara K;Satoh M
通讯作者：
Satoh M
影响因子：
4.8
作者：
Heimburg, Jamie;Yan, Jun;Rittenhouse-Olson, Kate
通讯作者：
Rittenhouse-Olson, Kate
影响因子：
3.6
作者：
Collin, Mattias;Ehlers, Marc
通讯作者：
Ehlers, Marc
影响因子：
2.8
作者：
Klaamas, K;Kurtenkov, O;Engstrand, L
通讯作者：
Engstrand, L
DOI：
10.1111/apm.1965.64.1.31
发表时间：
1965-01-01
期刊：
ACTA PATHOLOGICA ET MICROBIOLOGICA SCANDINAVICA
影响因子：
--
作者：
LAUREN, P
通讯作者：
LAUREN, P