Measurement of Taste Memory in Drosophila.
Measurement of Taste Memory in Drosophila.
复制标题
果蝇味觉记忆的测量。
DOI：
10.1101/pdb.prot108093
复制
发表时间：
2023
影响因子：
--
通讯作者：
Keene,AlexC
中科院分区：
文献类型：
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作者：
Brown,Elizabeth;Worden,Kurtresha;Li,Yuanyuan;Masek,Pavel;Keene,AlexC
由于先前的经验而改变行为的能力使生物体能够适应环境的变化。即使是天生的行为，如进食开始，也可以改变，如果以前与有害刺激有关。在这里，我们描述了一种味觉记忆测定配对食欲和苦味促味剂，导致厌恶的味觉条件反射。通过训练苍蝇将施加于跗骨的甜蔗糖与施加于长鼻的苦奎宁相关联，苍蝇很快学会抑制反射性长鼻向蔗糖的延伸，为行为和分子可塑性提供了生物测定。这种单蝇味觉记忆测定可应用于任何遗传背景的成年果蝇，并允许询问编码记忆的神经回路和分子过程，同时测量行为。与许多其他记忆测定不同，该系统需要很少的定制组件，因此可以在实验室中轻松建立，只需很少的苍蝇行为研究专业知识。
The ability to modify behavior as a result of previous experience allows an organism to adapt to changes in its environment. Even innate behaviors, like feeding initiation, can change if previously associated with a noxious stimulus. Here, we describe a taste memory assay pairing appetitive and bitter tastants, resulting in aversive taste conditioning. By training a fly to associate sweet sucrose applied to the tarsus with bitter quinine applied to the proboscis, flies quickly learn to suppress the reflexive proboscis extension to sucrose, providing a bioassay for behavioral and molecular plasticity. This single-fly taste memory assay may be applied to adult Drosophila of any genetic background and allows for interrogation of the neural circuitry and molecular processes encoding memories while simultaneously measuring behavior. Unlike many other memory assays, this system requires few custom components, and therefore can be easily established in laboratories with minimal expertise in the study of fly behavior.
果蝇反射性进食反应的测量。
影响因子：
--
作者：
Brown,Elizabeth;Worden,Kurtresha;Li,Yuanyuan;Masek,Pavel;Keene,AlexC
通讯作者：
Keene,AlexC