Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection
Chlamydophila felis CF0218 Is a Novel TMH Family Protein with Potential as a Diagnostic Antigen for Diagnosis of C. felis Infection
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猫衣原体 CF0218 是一种新型 TMH 家族蛋白，有可能作为诊断猫衣原体感染的诊断抗原
DOI：
10.1128/cvi.00134-08
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发表时间：
2008
影响因子：
--
通讯作者：
H. Fukushi
中科院分区：
文献类型：
--
作者：
K. Ohya;Y. Takahara;Etsuko Kuroda;S. Koyasu;S. Hagiwara;M. Sakamoto;Mitsuaki Hisaka;Kazuko Morizane;S. Ishiguro;Tsuyoshi Yamaguchi;H. Fukushi
摘要猫嗜衣原体是猫的急性和慢性结膜炎和肺炎（猫衣原体病）的病原体。此外，C.猫被怀疑是人类非沙眼衣原体结膜炎等疾病的人畜共患病原体，尽管这是有争议的。目前，还没有特异性检测C.猫的感染。目前的系统使用抗原，如脂多糖，与所有衣原体物种交叉反应。此外，很难区分接种了抗C的商业疫苗的猫。感染C.猫在这里，我们描述了一个新的候选诊断抗原诊断C。通过筛选猫衣原体基因组表达文库，获得了猫衣原体感染株CF 0218。felis Fe/C-56与C.猫免疫血清。CF 0218是一个假定的跨膜头（TMH）家族蛋白，在其N端具有双叶疏水基序，并且在肺炎衣原体或沙眼衣原体基因组中未发现CF 0218的直向同源物。重组CF 0218不能被抗C.提示CF 0218为沙眼衣原体。猫特有的CF 0218在C.逆转录-PCR证实猫感染。通过间接免疫荧光分析，CF 0218与C.感染细胞中的猫形成的包涵体。抗CF 0218的抗体应答在C.猫感染，但在实验接种和感染猫中未接种。这些结果表明，CF 0218，一个新的TMH家族蛋白C。felis，具有作为C.猫感染特异性诊断抗原
ABSTRACT Chlamydophila felis is a causative agent of acute and chronic conjunctivitis and pneumonia in cats (feline chlamydiosis). Also, C. felis is a suspected zoonotic agent of such diseases as non-Chlamydia trachomatis conjunctivitis in humans, although this is controversial. At present, there is no serodiagnostic system that specifically detects C. felis infection conveniently. Current systems use antigens such as lipopolysaccharide that cross-react with all chlamydia species. In addition, it is difficult to distinguish between cats that are vaccinated with the commercial vaccine against C. felis and cats that are infected with C. felis. Here, we describe a new candidate diagnostic antigen for diagnosis of C. felis infection, CF0218, that was obtained by screening a genomic expression library of C. felis Fe/C-56 with C. felis-immunized serum. CF0218 was a putative transmembrane head (TMH) family protein with bilobed hydrophobic motifs at its N terminus, and orthologues of CF0218 were not found in the Chlamydophila pneumoniae or Chlamydia trachomatis genomes. The recombinant CF0218 was not recognized by antiserum against C. trachomatis, suggesting that CF0218 is C. felis specific. CF0218 transcription during the course of C. felis infection was confirmed by reverse transcription-PCR. By indirect immunofluorescence analysis, CF0218 was colocalized with the C. felis-formed inclusion bodies in the infected cells. The antibody response against CF0218 was elevated following C. felis infection but not by vaccination in experimentally vaccinated and infected cats. These results suggest that CF0218, a novel TMH family protein of C. felis, possesses potential as a C. felis infection-specific diagnostic antigen.
影响因子：
56.9
作者：
Stephens, RS;Kalman, S;Davis, RW
通讯作者：
Davis, RW