Electrogenic arginine transport mediates stimulus‐secretion coupling in mouse pancreatic beta‐cells.
Electrogenic arginine transport mediates stimulus‐secretion coupling in mouse pancreatic beta‐cells.
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生电精氨酸转运介导小鼠胰腺β细胞中的刺激分泌耦合。
DOI：
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
F. Ashcroft
中科院分区：
文献类型：
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作者：
P. Smith;H. Sakura;B. Coles;N. Gummerson;P. Proks;F. Ashcroft
1.我们研究了L-精氨酸刺激膜去极化、细胞内钙（[Ca 2 +]i）增加和胰腺β细胞胰岛素分泌的机制。2.通过NAD（P）H自体荧光监测，L-精氨酸未能影响β细胞代谢。3. L-精氨酸产生[Ca 2 +]i的剂量依赖性增加，其依赖于膜去极化和细胞外钙。4.阳离子氨基酸L-鸟氨酸、L-赖氨酸、L-高精氨酸（未代谢）和NG-单甲基-L-精氨酸（L-NMMA，一种一氧化氮合酶抑制剂）产生的[Ca 2 +]i反应与L-精氨酸类似。中性一氧化氮合酶抑制剂NG-硝基-L-精氨酸（L-NNA）和N-ω-单甲基-L-精氨酸（L-NAME）也增加[Ca 2 +]i。D-精氨酸无效。5. L-精氨酸不影响全细胞Ca 2+电流或ATP敏感性K+电流，但产生由氨基酸携带的内向电流。6.逆转录酶-聚合酶链反应证明β细胞内存在鼠阳离子氨基酸转运蛋白mCAT 2A和mCAT 2B的信使RNA。7. L-精氨酸不影响β-细胞胞吐作用，如通过细胞电容的变化所测定的。8.我们的数据表明，L-精氨酸升高[Ca 2 +]i，并刺激胰岛素分泌，这是其进入β细胞的产电转运的结果。这种摄取由mCAT 2A转运蛋白介导。
1. We have investigated the mechanism by which L‐arginine stimulates membrane depolarization, an increase of intracellular calcium ([Ca2+]i) and insulin secretion in pancreatic beta‐cells. 2. L‐Arginine failed to affect beta‐cell metabolism, as monitored by NAD(P)H autofluorescence. 3. L‐Arginine produced a dose‐dependent increase in [Ca2+]i, which was dependent on membrane depolarization and extracellular calcium. 4. The cationic amino acids L‐ornithine, L‐lysine, L‐homoarginine (which is not metabolized) and NG‐monomethyl‐L‐arginine (L‐NMMA, a nitric oxide synthase inhibitor) produced [Ca2+]i responses similar to that produced by L‐arginine. The neutral nitric oxide synthase inhibitors NG‐nitro‐L‐arginine (L‐NNA) and N omega‐monomethyl‐L‐arginine (L‐NAME) also increased [Ca2+]i. D‐Arginine was ineffective. 5. L‐Arginine did not affect whole‐cell Ca2+ currents or ATP‐sensitive K+ currents, but produced an inward current that was carried by the amino acid. 6. The reverse transcriptase‐polymerase chain reaction demonstrated the presence of messenger RNA for the murine cationic amino acid transporters mCAT2A and mCAT2B within the beta‐cell. 7. L‐Arginine did not affect beta‐cell exocytosis as assayed by changes in cell capacitance. 8. Our data suggest that L‐arginine elevates [Ca2+]i and stimulates insulin secretion as a consequence of its electrogenic transport into the beta‐cell. This uptake is mediated by the mCAT2A transporter.
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DOI：
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发表时间：
1993
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The Journal of biological chemistry
影响因子：
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作者：
Closs,EI;Albritton,LM;Kim,JW;Cunningham,JM
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SCHMIDT, HHHW;WARNER, TD;MURAD, F
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