Low-cost multi-modal microscope using Raspberry Pi

Low-cost multi-modal microscope using Raspberry Pi
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使用 Raspberry Pi 的低成本多模式显微镜

DOI:
10.1016/j.ijleo.2020.164713
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发表时间:
2020
期刊:
影响因子:
3.1
通讯作者:
Y. Tamada
Y. Tamada
中科院分区:
物理与天体物理3区
文献类型:
--
作者:
W. Watanabe;Ryoji Maruyama;H. Arimoto;Y. Tamada

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本报告描述了一种使用Raspberry Pi操作的方向相关光学显微镜,其LED阵列照明模式由Raspberry Pi控制。图像是用Raspberry Pi相机模块获得的。我们展示了使用树莓派为基础的显微镜多模态成像,包括明场显微镜,暗场显微镜,微分相衬显微镜,和偏振显微镜。我们还演示了方向相关的暗场和差分相衬图像的采集。从一对相对的线或矩形照明,结构沿着照明轴增强暗场图像和微分相衬图像。Raspberry-Pi是开源硬件。基于Raspberry-Pi的紧凑型、具有成本效益的多对比度显微镜有望用于科学、技术、工程和数学(STEM)教育。
This report describes a direction-dependent optical microscope operated with Raspberry Pi, with LED array illumination patterns controlled by Raspberry Pi. Images are obtained with a Raspberry Pi camera module. We demonstrate the use of Raspberry-Pi-based microscopes for multimodal imaging including bright-field microscopy, dark-field microscopy, differential phase-contrast microscopy, and polarization microscopy. We also demonstrate acquisition of direction-dependent dark-field and differential phase-contrast images. From a pair of opposite line or rectangular illumination, structures along the illumination axis were enhanced in dark-field images and differential phase-contrast images. Raspberry-Pi is open-source hardware. Raspberry-Pi-based compact, cost-effective, and multi-contrast microscopes are expected to be useful for science, technology, engineering and mathematics (STEM) education.
100欧元的实验室:一个可在斑马鱼,果蝇和秀丽隐杆线虫行为过程中,用于荧光显微镜,光遗传学和准确温度控制的3D打印机平台。
DOI: 10.1371/journal.pbio.2002702
发表时间: 2017-07
期刊: PLoS biology
影响因子: 9.8
作者:
Maia Chagas A;Prieto-Godino LL;Arrenberg AB;Baden T
通讯作者: Baden T