Non-conventional octameric structure of C-phycocyanin.
Non-conventional octameric structure of C-phycocyanin.
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C-藻蓝蛋白的非常规八聚体结构。
DOI：
10.1038/s42003-021-02767-x
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发表时间：
2021-10-29
影响因子：
5.9
通讯作者：
Yoon KS
中科院分区：
文献类型：
--
作者：
Minato T;Teramoto T;Adachi N;Hung NK;Yamada K;Kawasaki M;Akutsu M;Moriya T;Senda T;Ogo S;Kakuta Y;Yoon KS
藻蓝蛋白（CPC）是一种蓝色色素蛋白，是巨大藻胆体的重要组成部分，藻胆体是蓝藻中捕光天线复合体，能有效地将能量传递给光系统I和II。X射线晶体学和电子显微镜（EM）分析表明，CPC的结构是一个封闭的环形六聚体组装两个三聚体。在这项研究中，非传统的八聚体CPC的结构表征首次报道。分析的晶体和低温EM结构的原生CPC从丝状嗜热蓝藻Thermoleptolyngbya sp. O-77出乎意料地说明了传统的六聚体和新的八聚体的共存。此外，一个不寻常的二聚体状态，观察到通过分析超离心，被假定为一个关键的中间体结构，在组装以前未观察到的八聚体。这些观察结果为CPC的组装过程和捕光复合物中的能量转移机制提供了新的见解。Takuo美娜多及其同事确定了来自嗜热蓝细菌Thermoleptolyngbya sp. O 77的天然C-藻蓝蛋白（CPC）的晶体和冷冻电镜结构，发现CPC采用传统的六聚体结构和新的八聚体组装。这些发现为CPC的组装及其能量传递机制提供了新的见解。
C-phycocyanin (CPC), a blue pigment protein, is an indispensable component of giant phycobilisomes, which are light-harvesting antenna complexes in cyanobacteria that transfer energy efficiently to photosystems I and II. X-ray crystallographic and electron microscopy (EM) analyses have revealed the structure of CPC to be a closed toroidal hexamer by assembling two trimers. In this study, the structural characterization of non-conventional octameric CPC is reported for the first time. Analyses of the crystal and cryogenic EM structures of the native CPC from filamentous thermophilic cyanobacterium Thermoleptolyngbya sp. O–77 unexpectedly illustrated the coexistence of conventional hexamer and novel octamer. In addition, an unusual dimeric state, observed via analytical ultracentrifugation, was postulated to be a key intermediate structure in the assemble of the previously unobserved octamer. These observations provide new insights into the assembly processes of CPCs and the mechanism of energy transfer in the light-harvesting complexes. Takuo Minato and colleagues determine the crystal and cryo-EM structures of the native C-phycocyanin (CPC) from the thermophilic cyanobacterium, Thermoleptolyngbya sp. O77, which was found to adopt both a conventional hexameric structure and a novel octameric assembly. These findings provide new insights into the assembly of CPCs and their mechanism of energy transfer.
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DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
5.6
作者：
David, Liron;Marx, Ailie;Adir, Noam
通讯作者：
Adir, Noam
影响因子：
3.7
作者：
Eisenberg, Ido;Harris, Dvir;Paltiel, Yossi
通讯作者：
Paltiel, Yossi
影响因子：
2.8
作者：
BRYANT, DA;GUGLIELMI, G;COHENBAZIRE, G
通讯作者：
COHENBAZIRE, G
DOI：
10.1107/s0907444909052925
发表时间：
2010-02
期刊：
Acta crystallographica. Section D, Biological crystallography
影响因子：
--
作者：
Adams PD;Afonine PV;Bunkóczi G;Chen VB;Davis IW;Echols N;Headd JJ;Hung LW;Kapral GJ;Grosse-Kunstleve RW;McCoy AJ;Moriarty NW;Oeffner R;Read RJ;Richardson DC;Richardson JS;Terwilliger TC;Zwart PH
通讯作者：
Zwart PH