IFN-gamma- and cell-to-cell contact-dependent cytotoxicity of allograft-induced macrophages against syngeneic tumor cells and cell lines: an application of allografting to cancer treatment.
IFN-gamma- and cell-to-cell contact-dependent cytotoxicity of allograft-induced macrophages against syngeneic tumor cells and cell lines: an application of allografting to cancer treatment.
复制标题
同种异体移植诱导的巨噬细胞对同基因肿瘤细胞和细胞系的 IFN-γ 和细胞间接触依赖性细胞毒性：同种异体移植在癌症治疗中的应用。
作者：
R. Yoshida;Y. Yoneda;M. Kuriyama;T. Kubota
在同种异体肿瘤或皮肤移植中，通过区分自体和非自体，破坏同种异体细胞的排斥过程使同系细胞保持完整。在这里，我们研究了在体外和体内，浸润到同种异体移植物中的细胞是否对同系永生细胞具有细胞毒性。白细胞（即，巨噬细胞（Mphi;在C57 BL/6小鼠中，浸润到同种异体移植物中但不浸润到自体移植物中的大量浸润细胞（55-65%）、粒细胞（20-25%）和淋巴细胞（15-20%）对同源肿瘤细胞和细胞系具有细胞毒性，而在同种异体移植的IFN-γ-/-C57 BL/6小鼠中几乎不诱导细胞毒性活性。在白细胞中，Mphi是细胞毒性细胞的主要群体;并且细胞毒性活性似乎是细胞与细胞接触依赖性的。当同源肿瘤细胞被s.c.与富含Mphi的细胞群或同种异体而非同基因的成纤维细胞同时注射到正常C57 BL/6小鼠中，肿瘤生长以细胞数量依赖性方式受到抑制，并且肿瘤细胞被排斥，Mphi：肿瘤比率约为30或同种异体移植物：肿瘤比率约为200。然而，在IFN-γ-/-C57 BL/6小鼠的情况下，s.c.同时注射同种异体移植物和肿瘤细胞对肿瘤生长没有影响。这些结果表明，同种异体移植物或同种异体移植物诱导的Mphi可能适用于癌症治疗，同种异体移植物诱导IFN-γ可能是治疗的关键。
In allogeneic tumor or skin transplantation, the rejection process that destroys the allogeneic cells leaves syngeneic cells intact by discrimination between self and nonself. Here, we examined whether the cells infiltrating into the allografts could be cytotoxic against syngeneic immortal cells in vitro and in vivo. The leukocytes (i.e., macrophages (Mphi; 55-65% of bulk infiltrates), granulocytes (20-25%), and lymphocytes (15-20%)) infiltrating into allografts, but not into autografts, in C57BL/6 mice were cytotoxic against syngeneic tumor cells and cell lines, whereas the cytotoxic activity was hardly induced in allografted, IFN-gamma-/- C57BL/6 mice. Among the leukocytes, Mphi were the major population of cytotoxic cells; and the cytotoxic activity appeared to be cell-to-cell contact dependent. When syngeneic tumor cells were s.c. injected into normal C57BL/6 mice simultaneously with the Mphi-rich population or allogeneic, but not syngeneic, fibroblastic cells, tumor growth was suppressed in a cell number-dependent manner, and tumor cells were rejected either with a Mphi:tumor ratio of about 30 or with an allograft:tumor ratio of approximately 200. In the case of IFN-gamma-/- C57BL/6 mice, however, the s.c. injection of the allograft simultaneously with tumor cells had no effect on the tumor growth. These results suggest that allograft or allograft-induced Mphi may be applicable for use in cancer treatment and that IFN-gamma induction by the allograft may be crucial for the treatment.
使用选择性清除 CD8 阳性 T 淋巴细胞保留移植物抗白血病，以预防慢性粒细胞白血病骨髓移植后的移植物抗宿主病。
影响因子：
0.9
作者：
Champlin,R;Jansen,J;Ho,W;Gajewski,J;Nimer,S;Lee,K;Territo,M;Winston,D;Tricot,G;Reichert,T
通讯作者：
Reichert,T
非溶细胞性 CD4(+) T 细胞群对心脏同种异体移植物的急性排斥。
影响因子：
6.2
作者：
VanBuskirk,AM;Wakely,ME;Orosz,CG
通讯作者：
Orosz,CG