A Genome Resequencing-Based Genetic Map Reveals the Recombination Landscape of an Outbred Parasitic Nematode in the Presence of Polyploidy and Polyandry.
A Genome Resequencing-Based Genetic Map Reveals the Recombination Landscape of an Outbred Parasitic Nematode in the Presence of Polyploidy and Polyandry.
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DOI：
10.1093/gbe/evx269
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发表时间：
2018-02-01
影响因子：
3.3
通讯作者：
Sargison ND
中科院分区：
文献类型：
--
作者：
Doyle SR;Laing R;Bartley DJ;Britton C;Chaudhry U;Gilleard JS;Holroyd N;Mable BK;Maitland K;Morrison AA;Tait A;Tracey A;Berriman M;Devaney E;Cotton JA;Sargison ND
寄生线虫捻转血矛线虫是小型反刍动物的一种经济和临床重要病原体，也是了解驱虫药抗性等性状机制和进化的模型系统。驱虫药耐药性普遍存在，是全球畜牧业可持续发展的主要威胁;然而，对基因组结构和参数（如重组）知之甚少，这些参数最终会影响耐药性的演变和传播速度。在这里，我们在两个不同的H菌株之间进行了遗传杂交。contortus，并随后使用全基因组重测序的雌性蠕虫和她的育雏，以确定分布的全基因组变异的特点，这些菌株。使用一种新的生物信息学方法来识别在假测交中按预期分离的变体，我们表征了连锁群并估计了标记之间的遗传距离，以生成染色体规模的F1遗传图谱。我们利用这张图来揭示重组景观，第一次为任何蠕虫物种，展示了广泛的染色体内和染色体之间的重组率的变化。对这些数据的分析还揭示了一妻多夫制的程度，其中至少有八名男性被发现有助于分析后代的遗传变异。还鉴定了三倍体后代，我们假设这是雌性减数分裂或多精受精过程中不分离的结果。这些结果扩展了我们对寄生蠕虫遗传学和H.我们还将继续努力，了解对用于控制这些蠕虫以及感染全世界牲畜和人类的相关物种的药物产生抗药性的遗传基础。本研究还证明了使用全基因组重测序数据直接构建遗传图谱的可行性，从一个非近交的非模式生物具有复杂的生命周期的单代杂交。
The parasitic nematode Haemonchus contortus is an economically and clinically important pathogen of small ruminants, and a model system for understanding the mechanisms and evolution of traits such as anthelmintic resistance. Anthelmintic resistance is widespread and is a major threat to the sustainability of livestock agriculture globally; however, little is known about the genome architecture and parameters such as recombination that will ultimately influence the rate at which resistance may evolve and spread. Here, we performed a genetic cross between two divergent strains of H. contortus, and subsequently used whole-genome resequencing of a female worm and her brood to identify the distribution of genome-wide variation that characterizes these strains. Using a novel bioinformatic approach to identify variants that segregate as expected in a pseudotestcross, we characterized linkage groups and estimated genetic distances between markers to generate a chromosome-scale F1 genetic map. We exploited this map to reveal the recombination landscape, the first for any helminth species, demonstrating extensive variation in recombination rate within and between chromosomes. Analyses of these data also revealed the extent of polyandry, whereby at least eight males were found to have contributed to the genetic variation of the progeny analyzed. Triploid offspring were also identified, which we hypothesize are the result of nondisjunction during female meiosis or polyspermy. These results expand our knowledge of the genetics of parasitic helminths and the unusual life-history of H. contortus, and enhance ongoing efforts to understand the genetic basis of resistance to the drugs used to control these worms and for related species that infect livestock and humans throughout the world. This study also demonstrates the feasibility of using whole-genome resequencing data to directly construct a genetic map in a single generation cross from a noninbred nonmodel organism with a complex lifecycle.
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影响因子：
30.8
作者：
Andersen, Erik C.;Gerke, Justin P.;Shapiro, Joshua A.;Crissman, Jonathan R.;Ghosh, Rajarshi;Bloom, Joshua S.;Felix, Marie-Anne;Kruglyak, Leonid
通讯作者：
Kruglyak, Leonid
影响因子：
4.5
作者：
Choi YJ;Bisset SA;Doyle SR;Hallsworth-Pepin K;Martin J;Grant WN;Mitreva M
通讯作者：
Mitreva M
影响因子：
2.6
作者：
Albertson, Donna G.;Thomson, J. Nichol
通讯作者：
Thomson, J. Nichol
影响因子：
7
作者：
Beye, Martin;Gattermeier, Irene;Page, Robert E., Jr.
通讯作者：
Page, Robert E., Jr.
影响因子：
4.5
作者：
Chan AH;Jenkins PA;Song YS
通讯作者：
Song YS