Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis.
Rapid Liquid AP-MALDI MS Profiling of Lipids and Proteins from Goat and Sheep Milk for Speciation and Colostrum Analysis.
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DOI：
10.3390/proteomes8030020
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发表时间：
2020-08-21
期刊：
影响因子：
3.3
通讯作者：
Cramer R
中科院分区：
文献类型：
--
作者：
Piras C;Ceniti C;Hartmane E;Costanzo N;Morittu VM;Roncada P;Britti D;Cramer R
牛奶生物分子成分的快速分析可用于食品质量评估和食品欺诈检测。来自特定物种的牛奶在商业价值和可获得性方面的差异往往是非法和欺诈性销售错误申报物种来源的牛奶的原因。在这项研究中，提出了一种快速的，基于MS的形态分析方法来区分绵羊从羊奶和羊初乳在不同阶段。使用液体大气压（AP）-基质辅助激光解吸/电离（MALDI）MS，可以在每个样品的一分钟数据采集中以100%的准确度对山羊和绵羊奶样品进行分类。此外，在纯羊奶样品和含有10%羊奶的羊奶样品中，分类准确率达到98%。评估初乳质量和产后阶段是这项技术的另一种可能的应用。对分娩后6 h内和分娩后48 h内采集的羊初乳样品进行分类，准确率为84.4%。我们的数据表明，脂质谱的实质性变化可以解释在早期和晚期时间点收集的初乳的准确分类。这种方法适用于不同物种的蛋白质直向同源物的分析，在这种情况下，允许明确的形态分析。
Rapid profiling of the biomolecular components of milk can be useful for food quality assessment and for food fraud detection. Differences in commercial value and availability of milk from specific species are often the reasons for the illicit and fraudulent sale of milk whose species origin is wrongly declared. In this study, a fast, MS-based speciation method is presented to distinguish sheep from goat milk and sheep colostrum at different phases. Using liquid atmospheric pressure (AP)-matrix-assisted laser desorption/ionisation (MALDI) MS, it was possible to classify samples of goat and sheep milk with 100% accuracy in one minute of data acquisition per sample. Moreover, an accuracy of 98% was achieved in classifying pure sheep milk samples and sheep milk samples containing 10% goat milk. Evaluating colostrum quality and postnatal stages represents another possible application of this technology. Classification of sheep colostrum samples that were collected within 6 hours after parturition and 48 hours later was achieved with an accuracy of 84.4%. Our data show that substantial changes in the lipid profile can account for the accurate classification of colostrum collected at the early and late time points. This method applied to the analysis of protein orthologs of different species can, as in this case, allow unequivocal speciation analysis.
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影响因子：
16.6
作者：
Cramer, Rainer;Pirkl, Alexander;Hillenkamp, Franz;Dreisewerd, Klaus
通讯作者：
Dreisewerd, Klaus
影响因子：
3.5
作者：
Bartier, A. L.;Windeyer, M. C.;Doepel, L.
通讯作者：
Doepel, L.
影响因子：
3.5
作者：
Contarini, G.;Povolo, M.;Degano, L.
通讯作者：
Degano, L.
影响因子：
4.1
作者：
Hale, Oliver J.;Morris, Michael;Cramer, Rainer
通讯作者：
Cramer, Rainer
牦牛初乳和瞬时奶的化学成分。
影响因子：
2.7
作者：
Cui, N.;Wen, P. C.;Ren, F. Z.
通讯作者：
Ren, F. Z.