Expression of a ribosomal protein gene in axillary buds of pea seedlings.
Expression of a ribosomal protein gene in axillary buds of pea seedlings.
复制标题
核糖体蛋白基因在豌豆幼苗腋芽中的表达。
DOI：
10.1104/pp.100.3.1494
复制
发表时间：
1992
期刊：
影响因子：
7.4
通讯作者：
I. Sussex
中科院分区：
文献类型：
--
作者：
J. P. Stafstrom;I. Sussex
豌豆（PisumsativumL.）cv Alaska）不生长，据说是休眠的。顶芽的去头促进这些腋芽的生长，然后以与顶芽相同的方式发育。我们以前表明，独特的蛋白质组在休眠和生长的芽中表达。在这里，我们描述了克隆，测序和表达的cDNA克隆（pGB 8），是同源的核糖体蛋白L27从大鼠。对应于该克隆的RNA在断头后3小时增加13倍，在12小时后达到约35倍的最大增强，并在稍后的时间保持在略微降低的水平。顶芽、根尖和伸长的节间也含有pGB 8 mRNA，但完全展开的小叶和完全伸长的节间不含有。原位杂交分析表明，pGB 8 mRNA增加，在所有部分的芽内1小时的断头。在适当的条件下，可以使正在生长的芽停止生长并进入休眠状态;这些芽随后可以再次生长。因此，芽有能力经历多个生长和休眠周期。RNA凝胶印迹显示，pGB 8表达降低到休眠水平，芽停止生长。然而，原位杂交实验表明，pGB 8的表达继续在生长芽水平的顶端分生组织2天后，它是减少在其余的芽。当用浓度>/=10 μ M的吲哚乙酸处理含有芽的培养茎时，芽生长和芽中pGB 8的表达受到抑制。
Axillary buds of intact pea seedlings (Pisum sativum L. cv Alaska) do not grow and are said to be dormant. Decapitation of the terminal bud promotes the growth of these axillary buds, which then develop in the same manner as terminal buds. We previously showed that unique sets of proteins are expressed in dormant and growing buds. Here we describe the cloning, sequencing, and expression of a cDNA clone (pGB8) that is homologous to ribosomal protein L27 from rat. RNA corresponding to this clone increases 13-fold 3 h after decapitation, reaches a maximum enhancement of about 35-fold after 12 h, and persists at slightly reduced levels at later times. Terminal buds, root apices, and elongating internodes also contain pGB8 mRNA but fully expanded leaflets and fully elongated internodes do not. In situ hybridization analysis demonstrates that pGB8 mRNA increases in all parts of the bud within 1 h of decapitation. Under appropriate conditions, growing buds can be made to stop growing and become dormant; these buds subsequently can grow again. Therefore, buds have the capacity to undergo multiple cycles of growth and dormancy. RNA gel blots show that pGB8 expression is reduced to dormancy levels as soon as buds stop growing. However, in situ hybridization experiments show that pGB8 expression continues at growing-bud levels in the apical meristem for 2 d after it is reduced in the rest of the bud. When cultured stems containing buds are treated with indoleacetic acid at concentrations >/=10 mum, bud growth and expression of pGB8 in the buds are inhibited.
花转变期间烟草芽尖表达基因的鉴定。
DOI：
10.1105/tpc.2.10.963
发表时间：
1990
期刊：
The Plant cell
影响因子：
--
作者：
Kelly,AJ;Zagotta,MT;White,RA;Chang,C;Meeks-Wagner,DR
通讯作者：
Meeks-Wagner,DR