Rectified directional sensing in long-range cell migration.
Rectified directional sensing in long-range cell migration.
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DOI：
10.1038/ncomms6367
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发表时间：
2014-11-06
影响因子：
16.6
通讯作者：
Sawai, Satoshi
中科院分区：
文献类型：
--
作者：
Nakajima, Akihiko;Ishihara, Shuji;Imoto, Daisuke;Sawai, Satoshi
如何整合空间和时间信息来确定细胞迁移的方向仍然知之甚少。在这里，通过精确的微流体仿真的动态化学引诱物波，我们表明，在Dictyosteopathy，定向运动以及激活的小鸟苷三磷酸酶Ras的前沿抑制时，随着时间的推移，化学引诱物的浓度降低。这种定向感知的“整流”仅发生在波速的中间范围内，并且不需要磷酸肌醇-3-激酶或F-肌动蛋白。从建模分析，我们表明，整流自然出现在一个单层的非相干前馈电路与零阶超灵敏度。所需的刺激时间窗口预测了接近Ras激活和细胞溶质中蛋白质典型的抑制剂扩散的定向感测响应的~5 s瞬态。我们认为，网骨藻细胞的能力，仅在波阵面移动是密切相关的适应性反应的整流结合局部激活和全球抑制。 细胞迁移受空间和时间信息的调节，但两者如何整合还不清楚。在这里，Nakajima等人使用动态微流体梯度来表明，当化学引诱物浓度随时间降低时，网骨藻前缘的Ras激活被抑制。
How spatial and temporal information are integrated to determine the direction of cell migration remains poorly understood. Here, by precise microfluidics emulation of dynamic chemoattractant waves, we demonstrate that, in Dictyostelium, directional movement as well as activation of small guanosine triphosphatase Ras at the leading edge is suppressed when the chemoattractant concentration is decreasing over time. This ‘rectification’ of directional sensing occurs only at an intermediate range of wave speed and does not require phosphoinositide-3-kinase or F-actin. From modelling analysis, we show that rectification arises naturally in a single-layered incoherent feedforward circuit with zero-order ultrasensitivity. The required stimulus time-window predicts ~5 s transient for directional sensing response close to Ras activation and inhibitor diffusion typical for protein in the cytosol. We suggest that the ability of Dictyostelium cells to move only in the wavefront is closely associated with rectification of adaptive response combined with local activation and global inhibition. Cell migration is regulated by spatial and temporal information, but how the two are integrated is not well understood. Here, Nakajima et al. use dynamic microfluidics gradients to show that Ras activation at the leading edge of Dictyostelium is suppressed when chemoattractant concentration decreases over time.
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影响因子：
7.3
作者：
Iglesias PA
通讯作者：
Iglesias PA
影响因子：
7.8
作者：
Kashikar, Nachiket D.;Alvarez, Luis;Kaupp, U. Benjamin
通讯作者：
Kaupp, U. Benjamin
影响因子：
--
作者：
Geiger, J;Wessels, D;Soll, DR
通讯作者：
Soll, DR
DOI：
10.1083/jcb.201001129
发表时间：
2010-07-26
期刊：
The Journal of cell biology
影响因子：
--
作者：
Cai H;Das S;Kamimura Y;Long Y;Parent CA;Devreotes PN
通讯作者：
Devreotes PN
影响因子：
11.8
作者：
Charest, Pascale G.;Shen, Zhouxin;Lakoduk, Ashley;Sasaki, Atsuo T.;Briggs, Steven P.;Firtel, Richard A.
通讯作者：
Firtel, Richard A.