A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain.
A Chlamydomonas outer arm dynein mutant with a truncated beta heavy chain.
复制标题
衣藻外臂动力蛋白突变体，具有截短的 β 重链。
DOI：
10.1083/jcb.122.3.653
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Kamiya,R
中科院分区：
文献类型：
--
作者：
Sakakibara,H;Takada,S;King,SM;Witman,GB;Kamiya,R
分离出具有异常外动力蛋白臂的衣藻 oda4 鞭毛突变体 (oda4-s7) 的新等位基因。与之前描述的缺乏所有三个（α、β和γ）外臂动力蛋白重链的oda4轴丝不同，oda4-s7轴丝含有α和γ重链以及分子量约为160 kD的新型肽。该肽与 mAb (18 beta B) 发生反应，mAb 可识别 β 重链 NH2 末端部分的表位。这些观察结果表明，该突变体具有截短的 β 重链，并且 β 重链的 NH2 末端部分对于外臂的稳定组装非常重要。在轴丝横截面的外臂平均电子显微镜图像中，突变的外臂缺少中间部分，产生分叉的外观。连同我们之前发现突变体 oda11 缺乏 α 重链和臂的最外部分（Sakakibara, H., D. R. Mitchell, and R. Kamiya. 1991. J. Cell Biol. 113:615-622)，该结果定义了轴丝横截面中三个外臂重链的大致位置。 oda4-s7的游泳速度为65+/-8微米/秒，接近缺少整个外臂的oda4（62+/-8微米/秒），但显着低于野生型（194+/-23微米/秒）和oda11（119+/-17微米/秒）的速度。因此，缺乏β重链对外臂功能的损害比缺乏α重链更严重，这表明α链和β链在外臂功能中发挥不同的作用。
A new allele of the Chlamydomonas oda4 flagellar mutant (oda4-s7) possessing abnormal outer dynein arms was isolated. Unlike the previously described oda4 axoneme lacking all three (alpha, beta, and gamma) outer-arm dynein heavy chains, the oda4-s7 axoneme contains the alpha and gamma heavy chains and a novel peptide with a molecular mass of approximately 160 kD. The peptide reacts with a mAb (18 beta B) that recognizes an epitope on the NH2-terminal part of the beta heavy chain. These observations indicate that this mutant has a truncated beta heavy chain, and that the NH2-terminal part of the beta heavy chain is important for the stable assembly of the outer arms. In averaged electron microscopic images of outer arms from cross sections of axonemes, the mutant outer arm lacks its mid-portion, producing a forked appearance. Together with our previous finding that the mutant oda11 lacks the alpha heavy chain and the outermost portion of the arm (Sakakibara, H., D. R. Mitchell, and R. Kamiya. 1991. J. Cell Biol. 113:615-622), this result defines the approximate locations of the three outer arm heavy chains in the axonemal cross section. The swimming velocity of oda4-s7 is 65 +/- 8 microns/s, close to that of oda4 which lacks the entire outer arm (62 +/- 8 microns/s) but significantly lower than the velocities of wild type (194 +/- 23 microns/s) and oda11 (119 +/- 17 microns/s). Thus, the lack of the beta heavy chain impairs outer-arm function more seriously than does the lack of the alpha heavy chain, suggesting that the alpha and beta chains play different roles in outer arm function.
血管生成因子及其测定：甲酰甲硫氨酰亮氨酰苯丙氨酸、二磷酸腺苷、肝素、铜和牛内皮刺激因子的活性。
影响因子：
3.1
作者：
B. Mcauslan;W. Reilly;G. N. Hannan;G. Gole
通讯作者：
G. Gole
联合化疗治疗晚期胃肠癌血清铜和铜蓝蛋白水平的变化
影响因子：
--
作者：
A. Scanni;M. Tomirotti;L. Licciardello;E. Annibali;M. Biraghi;M. Trovato;M. Fittipaldi;Paola Adamoli;Guglielmo Curtarelli
通讯作者：
Guglielmo Curtarelli
小鼠胚胎成纤维细胞表达铜蓝蛋白（一种血管生成糖蛋白）。
DOI：
10.1016/0006-291x(85)90565-0
发表时间：
1985
影响因子：
3.1
作者：
Chu,FF;Olden,K
通讯作者：
Olden,K