Inhibition of RabSB expression attenuates Ca2+-dependent exocytosis in rat anterior pituitary cells
Inhibition of RabSB expression attenuates Ca2+-dependent exocytosis in rat anterior pituitary cells
复制标题
抑制 RabSB 表达可减弱大鼠垂体前叶细胞中 Ca2 依赖性胞吐作用
作者：
P. Lledo;Philippe Verniert;J. Vincent;W. Mason;R. Zorec
Rab3家族的低分子量gtp结合蛋白（小G蛋白）被认为是囊泡交通的中心调节因子1,2，Rab3亚家族的蛋白（Rab3A， B， C和D） 3-6被认为与膜囊泡或颗粒与质膜发生胞外融合有关7 - 10。Rab3A在大脑中高表达，而Rab3B在大鼠垂体前叶中主要表达。我们在这里报道，使用全细胞膜片钳技术将Rab3B的反义寡核苷酸引入垂体细胞，特异性和可逆地阻断Rab3B的表达。我们发现钙依赖性胞外分泌被抑制而不影响内吞作用。针对Rab3A的反义寡核苷酸没有作用。我们的研究结果表明，Rab3B可能是一个关键的细胞内信号分子，可以控制垂体前叶细胞中其他钙依赖过程的下游胞吐。
LOW-MOLECULAR-WEIGHT GTP-binding proteins (small G proteins) of the Rab family have been proposed to act as central regulators of vesicular traffic1,2, and proteins of the Rab3 subfamily (Rab3A, B, C and D)3–6 are thought to be associated with membrane vesicles or granules undergoing exocytotic fusion with the plasma membrane7–10. Rab3A is highly expressed in brain11, whereas Rab3B is the major form found in rat anterior pituitary gland. We report here that antisense oligonucleotides against Rab3B, introduced into pituitary cells using the whole-cell patch clamp technique, specifically and reversibly block expression of Rab3B. We find that calcium-dependent exocytosis is inhibited without affecting endocytosis. Antisense oligonucleotides directed against Rab3A have no effect. Our results indicate that Rab3B is likely to be a key intracellular signalling molecule which can control exocytosis downstream of other calcium-dependent processes in anterior pituitary cells.
DOI：
10.1073/pnas.89.11.5049
发表时间：
1992-06-01
影响因子：
11.1
作者：
BALDINI, G;HOHL, T;LODISH, HF
通讯作者：
LODISH, HF