Effect of a Novel Stent on Re-Endothelialization, Platelet Adhesion, and Neointimal Formation.
Effect of a Novel Stent on Re-Endothelialization, Platelet Adhesion, and Neointimal Formation.
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新型支架对再内皮化、血小板粘附和新内膜形成的影响。
DOI：
10.5551/jat.31062
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发表时间：
2016-01
影响因子：
4.4
通讯作者：
周敏
中科院分区：
文献类型：
--
作者：
周敏
目的血管内皮细胞钙粘附素(VE-cadherin，VE-cadherin)由新生内皮细胞(OECs)特异性表达。两性离子支架具有良好的抗污性和良好的血液相容性。因此，我们假设抗VE-钙粘蛋白抗体包被的两性支架(VE-cad-Z支架)将促进再内皮化，减少新生内膜形成，并抗血栓。方法对VE-cad-Z支架进行体外血小板黏附试验、血小板活化试验和嗅鞘细胞捕获能力检测。观察VE-cad-Z支架对兔左颈总动脉(n=15)再内皮化、血栓抗性和新生内膜增生的影响。结果在体外，VE-cad-Z支架具有更好的抗血小板活性和嗅鞘细胞捕获能力(DNA浓度：297.23±22.71vs67.49±15.26 ng/µL，P<0.01)。在体内，VE-cad-Z支架的通畅率明显高于裸金属支架(15/15比12/15)，并显著减少了血小板黏附和新生内膜的形成(3天和30天时，新生内膜面积分别为1.13±0.05和1.00±0.05 mm(2)，P<0.01和3.04±0.11和1.05±0.06 mm(2)，P<0.01)；狭窄百分比分别为20.99±0.98比18.72±0.97，P<0.01和56.46±2.20比19.45±1.24，P<0.01)。结论VE-cad-Z支架能特异性捕获嗅鞘细胞，促进内皮愈合，减少血小板黏附和新生内膜形成。
AIM Vascular endothelial-cadherin (VE-cadherin) is specifically expressed by outgrowth endothelial cells (OECs). Zwitterionic stent showed high antifouling and excellent blood compatibility. Therefore, we hypothesized that anti-VE-cadherin antibody-coated zwitterionic stents (VE-cad-Z stents) would promote re-endothelialization, reduce neointimal formation, and resist thrombus. METHODS VE-cad-Z stents were examined using platelet adhesion test, platelet activation, and OEC capture ability in vitro. In vivo effect of VE-cad-Z stents on re-endothelialization, thrombus-resistance, and neointima hyperplasia was investigated in left common carotid arteries of rabbits (n=15). RESULTS In vitro, VE-cad-Z stents showed better platelet-resistance and OEC-capture ability (DNA concentration: 297.23±22.71 versus 67.49±15.26 ng/µL, P＜0.01). In vivo, VE-cad-Z stents exhibited better patency rate than bare metal stents (BMS) (15/15 versus 12/15), and it significantly reduced platelet adhesion and neointima formation (neointima area: 1.13±0.05 versus 1.00±0.05mm(2), P＜0.01 and 3.04±0.11versus 1.05±0.06mm(2), P＜0.01, at 3 and 30 days, respectively; % stenosis: 20.99±0.98 versus 18.72±0.97, P＜0.01 and 56.46±2.20 versus 19.45±1.24, P＜0.01, at 3 and 30 days, respectively). CONCLUSION These data suggested that VE-cad-Z stents could specifically capture OECs, consequently promote endothelial healing, and also reduce platelet adhesion and neointima formation.
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DOI：
10.1021/la049959g
发表时间：
2004-05
期刊：
Langmuir : the ACS journal of surfaces and colloids
影响因子：
--
作者：
Kwangmeyung Kim;K. Shin;Hyun-Kyoung Kim;Chulhee Kim;Y. Byun
通讯作者：
Kwangmeyung Kim;K. Shin;Hyun-Kyoung Kim;Chulhee Kim;Y. Byun
影响因子：
2.8
作者：
FERRANS, VJ;SPRAY, TL;ROBERTS, WC
通讯作者：
ROBERTS, WC
DOI：
--
发表时间：
--
期刊：
--
影响因子：
--
作者：
Han-Kwang Yang;B. Oh;Young‐Bae Park;Hyo‐Soo Kim;I. Oh;Tae-Youn Kim;Hyun-Jai Cho;Hyun-Jae Kang
通讯作者：
Han-Kwang Yang;B. Oh;Young‐Bae Park;Hyo‐Soo Kim;I. Oh;Tae-Youn Kim;Hyun-Jai Cho;Hyun-Jae Kang
影响因子：
5
作者：
Richardson MR;Yoder MC
通讯作者：
Yoder MC
影响因子：
37.8
作者：
Komatsu, R;Ueda, M;Becker, AE
通讯作者：
Becker, AE