Pentamines as substrate for human spermine oxidase.
Pentamines as substrate for human spermine oxidase.
复制标题
DOI：
10.1248/bpb.b12-00818
复制
发表时间：
2013
影响因子：
2
通讯作者：
Sugita Y
中科院分区：
文献类型：
--
作者：
Takao K;Shirahata A;Samejima K;Casero RA;Igarashi K;Sugita Y
Substrate activities of various linear polyamines to human spermine oxidase (hSMO) were investigated. The activities were evaluated by monitoring the amount of H2O2 released from sample polyamines by hSMO. H2O2 was measured by a HPLC method that analyzed fluorescent dimers derived from the oxidation of homovanillic acid in the presence of horseradish peroxidase. Six triamines were tested and were found not to be hSMO substrates. Of sixteen tetramines tested, spermine (Spm) was the most active substrate, followed by homospermine and N-butylated Spm. Pentamines showed a characteristic pattern of substrate activity. Of thirteen pentamines tested, 3343 showed higher substrate activity than Spm, and 4343 showed similar activity to Spm. The activities of the other pentamines were as follows: 3443, 4443, 4344, 3344, 4334, 4444, and 3334 (in decreasing order). Product amines released from these pentamines by hSMO were then analyzed by HPLC. Triamine was the only observed product, and the amount of triamine was nearly equivalent to that of released H2O2. A marked difference in the pH dependency curves between tetramines and pentamines suggested that hSMO favored reactions with a non-protonated secondary nitrogen at the cleavage site. The Km and Vmax values for Spm and 3343 at pH 7.0 and 9.0 were consistent with the higher substrate activity of 3343 compared to Spm, as well as with the concept of a non-protonated secondary nitrogen at the cleavage site being preferred, and 3343 was well degraded at a physiological pH by hSMO.
登录
查看更多内容
影响因子：
5.4
作者：
Murray-Stewart, Tracy;Wang, Yanlin;Casero, Robert A., Jr.
通讯作者：
Casero, Robert A., Jr.
影响因子：
2.8
作者：
Tzouros, M;Chesnov, S;Bigler, L
通讯作者：
Bigler, L
影响因子：
2.9
作者：
Adachi, Mariya S.;Juarez, Paul R.;Fitzpatrick, Paul F.
通讯作者：
Fitzpatrick, Paul F.
影响因子：
4.8
作者：
Hyvonen, Mervi T.;Keinanen, Tuomo A.;Janne, Juhani
通讯作者：
Janne, Juhani
DOI：
10.1042/bj20090598
发表时间：
2009-07-15
期刊：
The Biochemical journal
影响因子：
--
作者：
Casero RA;Pegg AE
通讯作者：
Pegg AE