Comparative transcriptome analysis of Anthurium "Albama" and its anthocyanin-loss mutant.
Comparative transcriptome analysis of Anthurium "Albama" and its anthocyanin-loss mutant.
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DOI：
10.1371/journal.pone.0119027
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Xu L
中科院分区：
文献类型：
--
作者：
Li Z;Wang J;Zhang X;Xu L
红掌是世界上最重要的热带观赏植物之一。红掌的交易价值在热带花卉中仅次于热带兰花。花苞是主要的观赏器官，花青素含量是花苞颜色变化的主要原因。了解花萼颜色的分子调控，有助于红掌新品种的培育。为了揭示红掌（Anthurium andraeanum ' alama）及其独特的花青素丢失突变体的基因表达差异，我们收集了野生型和突变体在花苞发育的两个阶段（花苞从鞘中伸出和花苞完全展开）的路径，并提取了总rna进行转录组分析。利用短读测序技术（Illumina），分别将野生型和突变型两个阶段的51,955,564,53,8222,224,54,221,990和52,276,418个测序原始reads从头组装成111,268个独特序列（unigenes），平均长度为652 bp。47,563个unigenes与Nr数据库中的序列有显著的匹配，32,768个unigenes与Swiss-Prot数据库中的已知蛋白质有显著的相似。28,350个和19,293个unigenes分别与KEGG和COG数据库中的现有序列具有显著的相似性。此外，对野生型和突变型以及两个不同发育阶段的差异表达基因进行了分析，表明大量基因的表达由于突变而发生了变化。综上所述，这些数据表明，Illumina测序允许从头转录组组装，并可以获得A. andraeanum野生型和花青素损失突变体之间的差异表达基因。AN2和UFGT的表达差异可能导致花青素损失突变。
Anthurium is one of the most important tropical ornamental plants in the world. The traded value of anthurium is second only to that of tropical orchids among the tropical flowers. The spathe is the main ornamental organ and its color variation mainly arises from anthocyanin contents. Understanding the molecular regulation of spathe color will accelerate new variety creation of anthurium. To announce gene expression differences between Anthurium andraeanum ‘Albama’ and its one unique anthocyanin-loss mutant, we collected spathes of the wild-type and the mutant from two stages in spathe development (the flower separates protrude from the sheath and the spathe is fully expanded) and extracted total RNAs for transcriptome profiling. Using short read sequencing technology (Illumina), 51,955,564, 53,822,224, 54,221,990 and 52,276,418 sequencing raw reads, respectively, for wild-type and mutant in the two stages were assembled de novo into 111,268 unique sequences (unigenes) with a mean length of 652 bp. 47,563 unigenes had significant hits to the sequences in the Nr database, and 32,768 unigenes showed significant similarity to known proteins in the Swiss-Prot database. 28,350 and 19,293 unigenes had significant similarity to existing sequences in the KEGG and COG databases, respectively. Further, analysis of differentially expressed genes in the comparison between wild-type and mutant and between the two different developmental stages was carried out, indicating that the expression of an extensive set of genes changed as the result of mutation. Taken together, these data demonstrated that the Illumina sequencing allowed de novo transcriptome assembly and could obtain differentially expressed genes between A. andraeanum wild-type and the anthocyanin-loss mutant. The expression differences of AN2 and UFGT might cause the anthocyanin-loss mutation.
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影响因子：
3.3
作者：
Sharma M;Cortes-Cruz M;Ahern KR;McMullen M;Brutnell TP;Chopra S
通讯作者：
Chopra S
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
影响因子：
11.6
作者：
Goodman, CD;Casati, P;Walbot, V
通讯作者：
Walbot, V
影响因子：
6.2
作者：
Chen, Wen-Huei;Hsu, Chi-Yin;Ger, Mang-Jye
通讯作者：
Ger, Mang-Jye
影响因子：
5.1
作者：
Matus, J. T.;Poupin, M. J.;Arce-Johnson, P.
通讯作者：
Arce-Johnson, P.