Shotgun proteomic analysis on the diapause and non-diapause eggs of domesticated silkworm Bombyx mori.
Shotgun proteomic analysis on the diapause and non-diapause eggs of domesticated silkworm Bombyx mori.
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家蚕滞育与非滞育卵的鸟枪法蛋白质组学分析
DOI：
10.1371/journal.pone.0060386
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Liu J
中科院分区：
文献类型：
--
作者：
Fan L;Lin J;Zhong Y;Liu J
为了阐明家蚕滞育的分子机制，有必要从蛋白质水平上研究滞育的分子基础。本文采用液相色谱-串联质谱（LC-MS/MS）技术，获得了家蚕滞育卵和非滞育卵的多肽谱图，并进行了生物信息学分析。在滞育卵和非滞育卵中分别鉴定出501和562个蛋白质，其中共有蛋白质309个。在这些蛋白质中，鉴定出3种主要贮藏蛋白（卵黄蛋白原前体、卵特异蛋白和低分子脂蛋白30 K前体），9种热休克蛋白（HSP 19.9、20.1、20.4、20.8、21.4、23.7、70、90-kDa热休克蛋白和热休克同源蛋白），37种代谢酶，22种核糖体蛋白。在滞育卵和非滞育卵中分别鉴定出192和253个独特蛋白，其中24个和48个具有功能注释，这些独特蛋白表明非滞育卵中的代谢、mRNA翻译和蛋白质合成可能比滞育卵中的更高。采用定量逆转录PCR（qRT-PCR）比较了两种卵中4种鉴定蛋白的相对mRNA水平，结果显示与蛋白表达量存在一定的不一致性。在滞育和非滞育卵中分别鉴定的502种蛋白质中的486种和562种蛋白质中的545种的GO特征是可用的。此外，京都基因和基因组百科全书（KEGG）途径分析表明，代谢，翻译和转录途径可能在非二层顶蛋在这个阶段更活跃。
To clarify the molecular mechanisms of silkworm diapause, it is necessary to investigate the molecular basis at protein level. Here, the spectra of peptides digested from silkworm diapause and non-diapause eggs were obtained from liquid chromatography tandem mass spectrometry (LC-MS/MS) and were analyzed by bioinformatics methods. A total of 501 and 562 proteins were identified from the diapause and non-diapause eggs respectively, of which 309 proteins were shared commonly. Among these common-expressed proteins, three main storage proteins (vitellogenin precursor, egg-specific protein and low molecular lipoprotein 30 K precursor), nine heat shock proteins (HSP19.9, 20.1, 20.4, 20.8, 21.4, 23.7, 70, 90-kDa heat shock protein and heat shock cognate protein), 37 metabolic enzymes, 22 ribosomal proteins were identified. There were 192 and 253 unique proteins identified in the diapause and non-diapause eggs respectively, of which 24 and 48 had functional annotations, these unique proteins indicated that the metabolism, translation of the mRNA and synthesis of proteins were potentially more highly represented in the non-dipause eggs than that in the diapause eggs. The relative mRNA levels of four identified proteins in the two kinds of eggs were also compared using quantitative reverse transcription PCR (qRT-PCR) and showed some inconsistencies with protein expression. GO signatures of 486 out of the 502 and 545 out of the 562 proteins identified in the diapause and non-diapause eggs respectively were available. In addition, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis showed the Metabolism, Translation and Transcription pathway were potentially more active in the non-dipause eggs at this stage.
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影响因子：
3.4
作者：
DASGUPTA, S;HOHMAN, TC;CARPER, D
通讯作者：
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影响因子：
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作者：
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作者：
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通讯作者：
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