Alteration of the gut fecal microbiome in children living with HIV on antiretroviral therapy in Yaounde, Cameroon.
Alteration of the gut fecal microbiome in children living with HIV on antiretroviral therapy in Yaounde, Cameroon.
复制标题
DOI：
10.1038/s41598-021-87368-8
复制
发表时间：
2021-04-07
影响因子：
4.6
通讯作者：
Nkenfou CN
中科院分区：
文献类型：
--
作者：
Abange WB;Martin C;Nanfack AJ;Yatchou LG;Nusbacher N;Nguedia CA;Kamga HG;Fokam J;Kennedy SP;Ndjolo A;Lozupone C;Nkenfou CN
多种因素，如免疫破坏，预防性复方新诺明和抗逆转录病毒治疗，可能会影响出生时感染艾滋病毒的儿童肠道微生物组的结构和功能。为了了解HIV感染是否改变了肠道微生物组，并将微生物组结构和功能的变化与免疫状态，病毒学反应和儿科ART方案联系起来，我们对来自喀麦隆国际大都市雅温得的87名HIV感染者和82名非暴露HIV阴性儿童的肠道微生物组进行了表征。我们发现，感染艾滋病毒的儿童肠道微生物组的α多样性显著降低，β多样性改变，这可能与CD 4 + T细胞计数或病毒载量无关。在感染HIV的儿童中，阿克曼氏菌和粪杆菌属的水平增加，埃希氏菌和其他γ变形菌的水平降低，以及其他差异。我们注意到种族/地理对观察到的肠道微生物组组成的影响，并且与非核苷类逆转录酶抑制剂相比，使用利托那韦增强蛋白酶抑制剂（PI/r）的ART的儿童的肠道微生物组组成与HIV阴性对照组的差异更大。进一步的研究调查这种改变的肠道微生物组在增加疾病易感性中的作用，对于通过以下途径获得HIV的个体是必要的：母婴传播
Multiple factors, such as immune disruption, prophylactic co-trimoxazole, and antiretroviral therapy, may influence the structure and function of the gut microbiome of children infected with HIV from birth. In order to understand whether HIV infection altered gut microbiome and to relate changes in microbiome structure and function to immune status, virological response and pediatric ART regimens, we characterized the gut microbiome of 87 HIV-infected and 82 non-exposed HIV-negative children from Yaounde, a cosmopolitan city in Cameroon. We found that children living with HIV had significantly lower alpha diversity in their gut microbiome and altered beta diversity that may not be related to CD4+ T cell count or viral load. There was an increased level of Akkermansia and Faecalibacterium genera and decreased level of Escherichia and other Gamma proteobacteria in children infected with HIV, among other differences. We noted an effect of ethnicity/geography on observed gut microbiome composition and that children on ritonavir-boosted protease inhibitor (PI/r)-based ART had gut microbiome composition that diverged more from HIV-negative controls compared to those on non-nucleoside reverse-transcriptase inhibitors-based ART. Further studies investigating the role of this altered gut microbiome in increased disease susceptibility are warranted for individuals who acquired HIV via mother-to-child transmission.
登录
查看更多内容
DOI：
10.1093/cid/ciz1186
发表时间：
2020-12-31
期刊：
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
影响因子：
--
作者：
D'Souza AW;Moodley-Govender E;Berla B;Kelkar T;Wang B;Sun X;Daniels B;Coutsoudis A;Trehan I;Dantas G
通讯作者：
Dantas G
影响因子：
4.6
作者：
Ling Z;Jin C;Xie T;Cheng Y;Li L;Wu N
通讯作者：
Wu N
影响因子：
6.4
作者：
Flygel, Trym T.;Sovershaeva, Evgeniya;Flaegstad, Trond
通讯作者：
Flaegstad, Trond
影响因子：
12.2
作者：
Lozupone, Catherine A.;Rhodes, Matthew E.;Palmer, Brent E.
通讯作者：
Palmer, Brent E.
影响因子：
17.1
作者：
Bourke, Claire D.;Gough, Ethan K.;Prendergast, Andrew J.
通讯作者：
Prendergast, Andrew J.