The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis.
The role played by cell-substrate interactions in the pathogenesis of osteoclast-mediated peri-implant osteolysis.
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细胞基底相互作用在破骨细胞介导的植入术骨溶解的发病机理中所起的作用。
DOI：
10.1186/ar1938
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发表时间：
2006
影响因子：
4.9
通讯作者：
Goldring SR
中科院分区：
文献类型：
--
作者：
Shen Z;Crotti TN;McHugh KP;Matsuzaki K;Gravallese EM;Bierbaum BE;Goldring SR
Prosthetic wear debris-induced peri-implant osteolysis is a major cause of aseptic loosening after total joint replacement. In this condition, wear particles released from the implant components induce a granulomatous inflammatory reaction at the interface between implant and adjacent bone, leading to progressive bone resorption and loss of fixation. The present study was undertaken to characterize definitively the phenotype of osteoclast-like cells associated with regions of peri-implant focal bone resorption and to compare the phenotypic features of these cells with those of mononucleated and multinucleated cells associated with polyethylene wear particles. Peri-implant tissues were obtained from patients undergoing hip revision surgery for aseptic loosening after total joint replacement. Cells were examined for the expression of several markers associated with the osteoclast phenotype using immunohistochemistry, histochemistry, and/or in situ hybridization. CD68 protein, a marker expressed by multiple macrophage lineage cell types, was detected in mononucleated and multinucleated cells associated with polyethylene particles and the bone surface. Cathepsin K and tartrate-resistant acid phosphatase were expressed highly in both mononucleated and multinucleated cells associated with the bone surface. Levels of expression were much lower in cells associated with polyethylene particles. High levels of β3 integrin protein were detected in cells in contact with bone. Multinucleated cells associated with polyethylene particles exhibited faint positive staining. Calcitonin receptor mRNA expression was detected solely in multinucleated cells present in resorption lacunae on the bone surface and was absent in cells associated with polyethylene particles. Our findings provide further evidence that cells expressing the full repertoire of osteoclast phenotypic markers are involved in the pathogenesis of peri-implant osteolysis after total joint replacement. They also demonstrate that foreign body giant cells, although believed to be phenotypically and functionally distinct from osteoclasts, express many osteoclast-associated genes and gene products. However, the levels and patterns of expression of these genes in the two cell types differ. We speculate that, in addition to the role of cytokines and growth factors, the substrate with which these cells interact plays a critical role in their differential phenotypic and functional properties.
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与骨吸收调节有关的破骨细胞功能抗原在生化上与玻染素受体有关。
DOI：
10.1083/jcb.109.4.1817
发表时间：
1989-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Davies J;Warwick J;Totty N;Philp R;Helfrich M;Horton M
通讯作者：
Horton M
影响因子：
6.2
作者：
Gowen, M;Lazner, F;Kola, I
通讯作者：
Kola, I
DOI：
10.1302/0301-620x.83b6.10905
发表时间：
2001-08-01
影响因子：
--
作者：
Haynes, DR;Crotti, TN;Findlay, DM
通讯作者：
Findlay, DM
影响因子：
6.2
作者：
Konttinen, YT;Takagi, M;Santavirta, S
通讯作者：
Santavirta, S
DOI：
10.2106/00004623-199306000-00007
发表时间：
1993-06-01
影响因子：
5.3
作者：
JIRANEK, WA;MACHADO, M;HARRIS, WH
通讯作者：
HARRIS, WH