Electrospray ionization tandem mass spectrometry of sodiated adducts of cholesteryl esters.
Electrospray ionization tandem mass spectrometry of sodiated adducts of cholesteryl esters.
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DOI：
10.1007/s11745-011-3609-2
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发表时间：
2011-12
期刊：
影响因子：
1.9
通讯作者：
Ford DA
中科院分区：
文献类型：
--
作者：
Bowden JA;Shao F;Albert CJ;Lally JW;Brown RJ;Procknow JD;Stephenson AH;Ford DA
胆固醇酯（CE）是重要的脂质储存分子。本研究表明，钠化加合物的CE分子物种形成的正离子，可以检测到在两个调查扫描模式，以及通过利用类特定的碎片在MS/MS扫描模式。CE的常见中性丢失是胆固醇（NL 368.5）丢失，可用于特异性定量组织CE分子种类。使用这种MS/MS技术，在与亚油酸（18：2）或花生四烯酸（20：4）孵育的小鼠单核细胞衍生的巨噬细胞（J774细胞）中定量CE分子种类。这些研究表明，花生四烯酸不仅被掺入CE池中，而且还被延长，导致巨噬细胞中22：4和24：4 CE分子物质的蓄积。此外，该技术用于定量喂食西方饮食的雌性小鼠血浆粗脂质提取物中存在的CE分子种类，与喂食正常食物饮食的雌性小鼠相比，这导致含有单不饱和脂肪酸的CE分子种类富集。最后，NL 368.5光谱揭示了含有多不饱和脂肪酸的CE分子物种的脂肪族脂肪酸残基的氧化。总之，这些研究表明，使用钠化加合物的CE结合直接输注电喷雾电离串联质谱快速定量生物样品中的CE分子种类的效用。
Cholesteryl esters (CE) are important lipid storage molecules. The present study demonstrates that sodiated adducts of CE molecular species form positive ions that can be detected in both survey scan mode as well as by exploiting class-specific fragmentation in MS/MS scan modes. A common neutral loss for CEs is the loss of cholestane (NL 368.5), which can be used to specifically quantify tissue CE molecular species. Using this MS/MS technique CE molecular species were quantified in mouse monocyte-derived macrophages (J774 cells) incubated with either linoleic (18:2) or arachidonic acid (20:4). These studies revealed that arachidonic acid was not only incorporated into the CE pool, but also was elongated resulting in the accumulation of 22:4 and 24:4 CE molecular species in macrophages. Additionally, this technique was used to quantify CE molecular species present in crude lipid extracts from plasma of female mice fed a Western diet, which led to an enrichment in CE molecular species containing monounsaturated fatty acids compared to female mice fed a normal chow diet. Last, NL 368.5 spectra revealed the oxidation of the aliphatic fatty acid residues of CE molecular species containing polyunsaturated fatty acids. Taken together, these studies demonstrate the utility of using sodiated adducts of CE in conjunction with direct infusion electrospray ionization tandem mass spectrometry to rapidly quantify CE molecular species in biological samples.
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影响因子：
2.9
作者：
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通讯作者：
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