Genome-wide copy number profiling of mouse neural stem cells during differentiation
Genome-wide copy number profiling of mouse neural stem cells during differentiation
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小鼠神经干细胞分化过程中的全基因组拷贝数分析
DOI：
10.1016/j.gdata.2015.04.021
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Meese E
中科院分区：
文献类型：
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作者：
Fischer U;Ludwig N;Keller A;Backes C;Meese E
越来越多的证据表明，基因扩增存在于神经干细胞和祖细胞分化过程中。我们使用阵列CGH来发现小鼠神经干细胞在使用TGF-β和FCS进行分化诱导的分化过程中的拷贝数变化，包括基因扩增和缺失。阵列数据以登录号GSE 35523保藏在GEO（Gene Expression Omnibus，NCBI）中。在这里，我们详细描述了细胞培养的特点和我们的TaqMan qPCR实验，以验证阵列CGH分析。关于小鼠中基因扩增的阵列-CGH实验的解释和与这些实验相关的扩增染色体区域的进一步详细分析由Fischer及其同事在Oncotarget（Fischer et al.，2015年）的报告。我们提供了额外的信息，在分化过程中删除的染色体区域，并给出了一个令人印象深刻的概述在分化诱导的时间线上的拷贝数变化。
There is growing evidence that gene amplifications were present in neural stem and progenitor cells during differentiation. We used array-CGH to discover copy number changes including gene amplifications and deletions during differentiation of mouse neural stem cells using TGF-ß and FCS for differentiation induction. Array data were deposited in GEO (Gene Expression Omnibus, NCBI) under accession number GSE35523. Here, we describe in detail the cell culture features and our TaqMan qPCR-experiments to validate the array-CGH analysis. Interpretation of array-CGH experiments regarding gene amplifications in mouse and further detailed analysis of amplified chromosome regions associated with these experiments were published by Fischer and colleagues in Oncotarget (Fischer et al., 2015). We provide additional information on deleted chromosome regions during differentiation and give an impressive overview on copy number changes during differentiation induction at a time line.
全基因组拷贝数分析以检测神经祖细胞中的基因扩增
影响因子：
--
作者：
Fischer U;Keller A;Backes C;Meese E
通讯作者：
Meese E