Characterization, development and exploitation of EST-derived microsatellites in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
Characterization, development and exploitation of EST-derived microsatellites in Porphyra haitanensis Chang et Zheng (Bangiales, Rhodophyta)
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坛紫菜 (Bangiales，红藻门) EST 衍生微卫星的表征、开发和利用
DOI：
10.1007/s10811-008-9379-4
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发表时间：
2009-06
影响因子：
3.3
通讯作者：
谢潮添
中科院分区：
文献类型：
--
作者：
谢潮添
The frequency, type and distribution of simple sequence repeats (SSRs) in Porphyra haitanensis genomes was investigated using expressed sequence tag (EST) data deposited in public databases. A total of 3,489 non-redundant P. haitanensis ESTs were screened for SSRs using SSRhunter software. From those, 224 SSRs in 210 ESTs were identified; trinucleotides were the most common type of SSR (64.29%), followed by dinucleotides (33.48%). Tetranucleotides, pentanucleotides, and hexanucleotides were not common. Among all identified motif types, CGG/CCG had the highest frequency (33.9%), followed by TC/AG (24.6%). From these EST-SSRs, 37 SSR primer-pairs were designed and tested using common SSR reaction conditions with 15 P. haitanensis DNAs as templates. The results showed that 28 SSR primer-pairs gave good amplification patterns. These were used to conduct SSR analyses of genetic variations of the 15 germplasm strains of P. haitanensis. A total of 224 alleles were detected, with the number of alleles ranging from 4 to 15. The effective number of alleles, expected heterozygosity, and polymorphism information content of the 15 germplasm strains of P. haitanensis were 2.81, 0.64, and 0.57, respectively. All of these parameters indicate that the 15 germplasm strains of P. haitanensis harbor rich genetic variation.
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DOI：
--
发表时间：
2005-09
期刊：
Yi chuan = Hereditas
影响因子：
--
作者：
Qiang Li;J. Wan
通讯作者：
Qiang Li;J. Wan
DOI：
--
发表时间：
1987
期刊：
--
影响因子：
--
作者：
J. Hamrick
通讯作者：
J. Hamrick
影响因子：
2.9
作者：
Fan Xiaolei;Fang Yongjun;Wang Guangce
通讯作者：
Wang Guangce
DOI：
--
发表时间：
2005
期刊：
--
影响因子：
--
作者：
L. Bi
通讯作者：
L. Bi
影响因子：
3.3
作者：
L. Cardle;L. Ramsay;D. Milbourne;M. Macaulay;D. Marshall;R. Waugh
通讯作者：
L. Cardle;L. Ramsay;D. Milbourne;M. Macaulay;D. Marshall;R. Waugh