Alpha lipoic acid selectively inhibits proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells.
Alpha lipoic acid selectively inhibits proliferation and adhesion to fibronectin of v-H-ras-transformed 3Y1 cells.
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α脂酸选择性地抑制V-H-RAS转化的3Y1细胞的纤连蛋白的增殖和粘附。
DOI：
10.3164/jcbn.11-67
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发表时间：
2012-05
影响因子：
2.4
通讯作者：
Nishiyama K
中科院分区：
文献类型：
--
作者：
Yamasaki M;Iwase M;Kawano K;Sakakibara Y;Suiko M;Nishiyama K
本研究主要研究了外消旋α-硫辛酸对大鼠成纤维细胞3 Y1和v-H-ras转化衍生物HR-3 Y1 -2细胞增殖和粘附特性的影响。外消旋α-硫辛酸在0.3和1.0 mM时抑制HR-3 Y1 -2细胞的增殖，但不抑制3 Y1细胞的增殖。R-（+）-α-硫辛酸对HR-3 Y1 -2细胞增殖的抑制作用与外消旋α-硫辛酸相当。此外，外消旋α-硫辛酸降低HR-3 Y1细胞的细胞内活性氧水平，但对3 Y1细胞无影响。接下来，我们评估了外消旋α-硫辛酸对细胞粘附至纤连蛋白的影响。结果表明，外消旋α-硫辛酸可降低HR-3 Y1 -2细胞对纤连蛋白包被平板的粘附能力。由于阻断抗体实验表明β1-整合素在本实验系统中的细胞粘附中起关键作用，因此检测了外消旋α-硫辛酸对β1-整合素表达的影响。结果表明，外消旋α-硫辛酸可选择性下调HR-3 Y1 -2细胞表面β1-整合素的表达。有趣的是，在3 Y1细胞中，外源性过氧化氢上调细胞表面β1-整合素的表达。总之，这些数据表明，α-硫辛酸降低细胞内活性氧水平可能是改善v-H-ras转化细胞异常生长和粘附特性的有效手段。
Here, we focused on the effects of racemic α-lipoic acid on proliferation and adhesion properties of 3Y1 rat fibroblasts and the v-H-ras-transformed derivative, HR-3Y1-2 cells. Racemic α-lipoic acid inhibited proliferation of HR-3Y1-2 but not 3Y1 cells at 0.3 and 1.0 mM. R-(+)-α-lipoic acid also inhibited proliferation of HR-3Y1-2 cells equivalent to that of racemic α-lipoic acid. In addition, racemic α-lipoic acid decreased intracellular reactive oxygen species levels in HR-3Y1 cells but not 3Y1 cells. Next, we evaluated the effects of racemic α-lipoic acid on cell adhesion to fibronectin. The results indicated that racemic α-lipoic acid decreased adhesive ability of HR-3Y1-2 cells to fibronectin-coated plates. As blocking antibody experiment revealed that β1-integrin plays a key role in cell adhesion in this experimental system, the effects of racemic α-lipoic acid on the expression of β1-integrin were examined. The results indicated that racemic α-lipoic acid selectively downregulated the expression of cell surface β1-integrin expression in HR-3Y1-2 cells. Intriguingly, exogenous hydrogen peroxide upregulated cell surface β1-integrin expression in 3Y1 cells. Taken together, these data suggest that reduction of intracellular reactive oxygen species levels by α-lipoic acid could be an effective means of ameliorating abnormal growth and adhesive properties in v-H-ras transformed cells.
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影响因子：
8.8
作者：
Bamford, S;Dawson, E;Forbes, S;Clements, J;Pettett, R;Dogan, A;Flanagan, A;Teague, J;Futreal, PA;Stratton, MR;Wooster, R
通讯作者：
Wooster, R
影响因子：
4.4
作者：
Packer, L;Kraemer, K;Rimbach, G
通讯作者：
Rimbach, G
影响因子：
5.6
作者：
Traore, Kassim;Sharma, Rajni;Trush, Michael A.
通讯作者：
Trush, Michael A.
影响因子：
6.1
作者：
Li Chun-jun;Zhang Qiu-mei;Yu De-min
通讯作者：
Yu De-min
DOI：
10.1080/j157v05n03_05
发表时间：
2005-01-01
期刊：
Journal of Herbal Pharmacotherapy
影响因子：
--
作者：
Smith, Jerry Robert;Thiagaraj, Harish V.;Parker, Keith K.
通讯作者：
Parker, Keith K.