Disrupting microtubule network immobilizes amoeboid chemotactic receptor in the plasma membrane.
Disrupting microtubule network immobilizes amoeboid chemotactic receptor in the plasma membrane.
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DOI：
10.1016/j.bbamem.2011.02.009
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发表时间：
2011-06
影响因子：
3.4
通讯作者：
Iglesias, P. A.
中科院分区：
文献类型：
--
作者：
de Keijzer, S.;Galloway, J.;Harms, G. S.;Devreotes, P. N.;Iglesias, P. A.
关键词：
质膜上的信号级联是通过一个分子被另一个分子激活而启动的。这种相互作用取决于分子之间的相互作用，这取决于它们在细胞膜中的定位和横向扩散。细胞骨架通过增强或限制信号伙伴在质膜上相遇的可能性，在这一过程中发挥着非常重要的作用。在这项研究中，我们探索了cAMP受体cAR1在盘基网柄菌细胞质膜中的扩散方式，以及细胞骨架是如何调节这一扩散的。用全内反射显微镜对荧光标记的cAR1进行单粒子跟踪，发现70%的cAR1分子是可移动的。这些受体表现出定向运动，我们证明这不是因为沿着肌动蛋白细胞骨架的跟踪。相反，微管的不稳定取消了质膜中cAR1的流动性，这一点在光漂白后的荧光恢复中得到了证实。作为微管稳定的结果，第一个下游信号事件之一，CRAC的PH结构域的跳跃减少。这些结果表明，微管在cAR1动力学和cAR1分子与其信号伙伴相互作用的能力中发挥了作用。
Signaling cascades are initiated in the plasma membrane via activation of one molecule by another. The interaction depends on the mutual availability of the molecules to each other and this is determined by their localization and lateral diffusion in the cell membrane. The cytoskeleton plays a very important role in this process by enhancing or restricting the possibility of the signaling partners to meet in the plasma membrane. In this study we explored the mode of diffusion of the cAMP receptor, cAR1, in the plasma membrane of Dictyostelium discoideum cells and how this is regulated by the cytoskeleton. Single-particle tracking of fluorescently labeled cAR1 using total internal reflection microscopy showed that 70% of the cAR1 molecules were mobile. These receptors showed directed motion and we demonstrate that this is not because of tracking along the actin cytoskeleton. Instead, destabilization of the microtubules abolished cAR1 mobility in the plasma membrane and this was confirmed by fluorescence recovery after photobleaching. As a result of microtubule stabilization, one of the first downstream signaling events, the jump of the PH domain of CRAC, was decreased. These results suggest a role for microtubules in cAR1 dynamics and in the ability of cAR1 molecules to interact with their signaling partners.
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影响因子：
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