Insights into the transposable mobilome of Paracoccus spp. (Alphaproteobacteria).
Insights into the transposable mobilome of Paracoccus spp. (Alphaproteobacteria).
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DOI：
10.1371/journal.pone.0032277
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Bartosik D
中科院分区：
文献类型：
--
作者：
Dziewit L;Baj J;Szuplewska M;Maj A;Tabin M;Czyzkowska A;Skrzypczyk G;Adamczuk M;Sitarek T;Stawinski P;Tudek A;Wanasz K;Wardal E;Piechucka E;Bartosik D
使用几个TRAP质粒(能够对转座事件进行正向选择)来鉴定驻留在副球藻属(甲蛋白杆菌)细菌中的功能性转座元件(TES)池。对代表该属20种的25株菌株的复杂分析导致捕获和鉴定了(I)37个插入序列(ISS)，代表9个IS家族(IS3、IS5、IS6、IS21、IS66、IS256、IS1182、IS1380和IS1634)；(Ii)由ISPfe2(IS1634家族)的两个拷贝产生的复合转座子Tn6097，包含两个预测的遗传模块，参与精氨酸脱亚胺酶途径和柔红霉素/阿霉素抗性；(Iii)Tn3家族的3个非复合转座子，包括携带链霉素抗性的Tn5393和(Iv)一个可转座岛TnP1(45kb)。一些元件(例如IS5家族IS903组的Tn5393、Tn6097和ISS)被证明含有能够驱动位于转座靶点下游的基因转录的强启动子。通过应用含有无启动子四环素抗性报告基因的TRAP载体pCM132TC，我们鉴定了转座可以为转录沉默基因提供启动子的五种方式。除了强调几种TES的多样性和特异性外，本研究中进行的分析还提供了关于(I)转座过程的动力学(例如TnPpa1的转座频率异常高)和(Ii)转座介导的DNA结构变化(例如IS21家族的ISPve1转座时在受体分子中产生大的缺失)的新颖和有趣的信息。我们还展示了TES和转座在产生不同表型以及通过水平基因转移自然放大和传播遗传信息(具有适应价值)方面的巨大潜力，这被认为是细菌进化的驱动力。
Several trap plasmids (enabling positive selection of transposition events) were used to identify a pool of functional transposable elements (TEs) residing in bacteria of the genus Paracoccus (Alphaproteobacteria). Complex analysis of 25 strains representing 20 species of this genus led to the capture and characterization of (i) 37 insertion sequences (ISs) representing 9 IS families (IS3, IS5, IS6, IS21, IS66, IS256, IS1182, IS1380 and IS1634), (ii) a composite transposon Tn6097 generated by two copies of the ISPfe2 (IS1634 family) containing two predicted genetic modules, involved in the arginine deiminase pathway and daunorubicin/doxorubicin resistance, (iii) 3 non-composite transposons of the Tn3 family, including Tn5393 carrying streptomycin resistance and (iv) a transposable genomic island TnPpa1 (45 kb). Some of the elements (e.g. Tn5393, Tn6097 and ISs of the IS903 group of the IS5 family) were shown to contain strong promoters able to drive transcription of genes placed downstream of the target site of transposition. Through the application of trap plasmid pCM132TC, containing a promoterless tetracycline resistance reporter gene, we identified five ways in which transposition can supply promoters to transcriptionally silent genes. Besides highlighting the diversity and specific features of several TEs, the analyses performed in this study have provided novel and interesting information on (i) the dynamics of the process of transposition (e.g. the unusually high frequency of transposition of TnPpa1) and (ii) structural changes in DNA mediated by transposition (e.g. the generation of large deletions in the recipient molecule upon transposition of ISPve1 of the IS21 family). We also demonstrated the great potential of TEs and transposition in the generation of diverse phenotypes as well as in the natural amplification and dissemination of genetic information (of adaptative value) by horizontal gene transfer, which is considered the driving force of bacterial evolution.
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DOI：
10.1099/00207713-48-2-543
发表时间：
1998-04-01
期刊：
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY
影响因子：
--
作者：
Harker, M;Hirschberg, J;Oren, A
通讯作者：
Oren, A
影响因子：
2.8
作者：
Bartosik, D;Baj, J;Wlodarczyk, M
通讯作者：
Wlodarczyk, M
影响因子：
2.6
作者：
CAMPBELL, A;BERG, DE;SZYBALSKI, W
通讯作者：
SZYBALSKI, W
影响因子：
64.5
作者：
FRIEDMAN, DI
通讯作者：
FRIEDMAN, DI
影响因子：
3.6
作者：
De Palmenaer, D;Vermeiren, C;Mahillon, J
通讯作者：
Mahillon, J