Th1/Th17 polarization and acquisition of an arthritogenic phenotype in arthritis-susceptible BALB/c, but not in MHC-matched, arthritis-resistant DBA/2 mice.
Th1/Th17 polarization and acquisition of an arthritogenic phenotype in arthritis-susceptible BALB/c, but not in MHC-matched, arthritis-resistant DBA/2 mice.
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TH1/TH17在受关节炎敏感的BALB/C中的关节炎表型的极化和获取，但在MHC匹配的，具有关节炎的DBA/2小鼠中却不是。
DOI：
10.1093/intimm/dxp018
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发表时间：
2009-05
影响因子：
4.4
通讯作者：
Glant TT
中科院分区：
文献类型：
--
作者：
Boldizsar F;Tarjanyi O;Nemeth P;Mikecz K;Glant TT
蛋白聚糖（PG）聚集蛋白聚糖诱导的关节炎（PGIA）是类风湿性关节炎（RA）的鼠模型。虽然BALB/c和DBA/2小鼠共享相同的MHC（H-2d）单倍型，但BALB/c品系对PGIA敏感，而DBA/2小鼠对PGIA抵抗。因此，这两个近交系小鼠品系提供了一个机会，研究关节炎的易感因素，排除MHC相关的遗传成分的影响。本研究的目的是监测腹膜内（i. p.）免疫诱导PGIA;此外，我们试图通过比较BALB/c和DBA/2小鼠中PG诱导的免疫应答来鉴定新的易感因素。腹膜内注射PG后，BALB/c小鼠腹膜中的常驻幼稚B1细胞被T细胞和常规B细胞两者替代。这些腹膜T细胞产生IFNγ和IL-17，这些细胞因子在RA和相应的关节炎模型中显示出重要性。此外，腹膜细胞可以过继转移PGIA到SCID小鼠，证明其致关节炎特性。我们的研究结果表明，反复注射抗原导致腹膜中免疫细胞的募集和激活;这些细胞然后触发疾病的效应期。Th 1/Th 17细胞在腹腔内的迁移和活化可能是BALB/c小鼠关节炎易感性的关键因素。
Proteoglycan (PG) aggrecan-induced arthritis (PGIA) is a murine model of rheumatoid arthritis (RA). Although BALB/c and DBA/2 mice share the same MHC (H-2d) haplotype, the BALB/c strain is susceptible to PGIA, while DBA/2 mice are resistant. Therefore, these two inbred mouse strains provide an opportunity to study arthritis susceptibility factors excluding the effects of MHC-associated genetic components. The goal of this study was to monitor changes in the cellular composition and activation state following intra-peritoneal (i.p.) immunization to induce PGIA; additionally, we sought to identify new susceptibility factors by comparing PG-induced immune responses in BALB/c and DBA/2 mice. Upon i.p. PG injection, resident naive B1 cells are replaced by both T cells and conventional B cells in the peritoneum of BALB/c mice. These peritoneal T cells produce IFNγ and IL-17, cytokines shown to be important in RA and corresponding arthritis models. Moreover, peritoneal cells can adoptively transfer PGIA to SCID mice, demonstrating their arthritogenic properties. Our results indicate that repeatedly injected antigen leads to the recruitment and activation of immune cells in the peritoneum; these cells then trigger the effector phase of the disease. The migration and activation of Th1/Th17 cells in the peritoneal cavity in response to PG immunization, which did not occur in the arthritis-resistant DBA/2 strain, may be critical factors of arthritis susceptibility in BALB/c mice.
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影响因子：
4.4
作者：
Doodes, Paul D.;Cao, Yanxia;Hamel, Keith M.;Wang, Yumei;Farkas, Balint;Iwakura, Yoichiro;Finnegan, Alison
通讯作者：
Finnegan, Alison
影响因子：
--
作者：
Berlo, Suzanne E.;Guichelaar, Teun;Glant, Tibor T.
通讯作者：
Glant, Tibor T.
影响因子：
5.5
作者：
Klinguer-Hamour, C;Libon, C;Beck, A
通讯作者：
Beck, A
影响因子：
--
作者：
Hanyecz, A;Berlo, SE;Glant, TT
通讯作者：
Glant, TT
影响因子：
4.3
作者：
GRUN, JL;MAURER, PH
通讯作者：
MAURER, PH