Hypoxia Relieves X-Ray-Induced Delayed Effects in Normal Human Embryo Cells
Hypoxia Relieves X-Ray-Induced Delayed Effects in Normal Human Embryo Cells
复制标题
缺氧可缓解 X 射线引起的正常人类胚胎细胞的延迟效应
作者：
Kanaklata Roy;S. Kodama;Keiji Suzuki;K. Fukase;M. Watanabe
摘要 Roy, K.、Kodama, S.、Suzuki, K.、Fukase, K. 和 Watanabe, M. 缺氧可缓解正常人胚胎细胞中 X 射线引起的延迟效应。我们研究了缺氧对正常人胚胎细胞中 X 射线诱导的延迟效应的影响，以阐明氧化应激在细胞对辐射诱导遗传不稳定性的易感性中的作用。我们在正常含氧 (20%) 和缺氧 (2%) 条件下，在辐射后 28-38 倍群体倍增时，检查了 X 射线诱导的延迟细胞死亡、巨细胞形成和染色体畸变。结果显示，缺氧减少了 X 射线引起的延迟效应，表明辐射增强了细胞氧化应激，这在确定受辐射细胞对遗传不稳定性的易感性方面发挥着重要作用。本研究强调了表观遗传效应的生物学意义，例如氧张力，以及辐射诱导遗传不稳定性中的直接 DNA 损伤。
Abstract Roy, K., Kodama, S., Suzuki, K., Fukase, K. and Watanabe, M. Hypoxia Relieves X-Ray-Induced Delayed Effects in Normal Human Embryo Cells. We studied the effect of hypoxia on X-ray-induced delayed effects in normal human embryo cells to elucidate the role of oxidative stress in the susceptibility of cells to induction of genetic instability by radiation. We examined X-ray-induced delayed cell death, giant cell formation, and chromosome aberrations under normally oxygenated (20%) and hypoxic (2%) conditions at 28–38 population doublings postirradiation. The results revealed that hypoxia reduced the X-ray-induced delayed effects, suggesting that radiation enhances cellular oxidative stress, which plays a significant role in determining the susceptibility of irradiated cells to genetic instability. The present study emphasizes the biological significance of epigenetic effects, such as oxygen tension, as well as direct DNA damage in the induction of genetic instability by radiation.
DOI：
10.1073/pnas.77.12.7262
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
KENNEDY, AR;FOX, M;LITTLE, JB
通讯作者：
LITTLE, JB
DOI：
10.1073/pnas.96.9.4959
发表时间：
1999-04-27
影响因子：
11.1
作者：
Wu, LJ;Randers-Pehrson, G;Hei, TK
通讯作者：
Hei, TK
影响因子：
3.4
作者：
Little, JB;Nagasawa, H;Vetrovs, H
通讯作者：
Vetrovs, H