Contractility and ultrastructure in glycerol--extracted muscle fibers. II. Ultrastructure in resting and shortened fibers.

Contractility and ultrastructure in glycerol--extracted muscle fibers. II. Ultrastructure in resting and shortened fibers.
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DOI:
10.1083/jcb.27.1.35
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发表时间:
1965-10
期刊:
The Journal of cell biology
影响因子:
--
通讯作者:
Knappeis GG
Knappeis GG
中科院分区:
其他
文献类型:
--
作者:
Carlsen F;Fuchs F;Knappeis GG

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甘油提取的兔腰大肌肌纤维在ATP诱导的等张缩短之前和之后通过电子显微镜检查。超微结构变化与肌节起始长度和缩短程度相关。静息纤维在静息长度时的超微结构与H. E.赫胥黎和汉森。当肌节长度大于3.7至3.8 µ时,A和I丝分离并被间隙分开。“间隙”细丝的存在得到证实,并提出证据表明,这些细丝之间的A和I细丝的端部形成连接。从最初的肌节长度缩短在细丝重叠发生通过滑动的细丝。如果缩短是从肌节长度开始的,在那里有一个间隙,I带的狭窄是由A带和I带之间边界处的I丝卷曲引起的。没有证据表明I丝进入A带。
Glycerol-extracted rabbit psoas muscle fibers were examined by electron microscopy both before and after ATP-induced isotonic shortening. Ultrastructural changes were correlated with the initial sarcomere length and the degree of shortening. The ultrastructural appearance of the resting fiber at rest length was identical with that described by H. E. Huxley and Hanson. At sarcomere lengths greater than 3.7 to 3.8 µ, the A and I filaments were detached and separated by a gap. The presence of "gap" filaments was confirmed, and evidence is presented which indicates that these filaments form connections between the ends of the A and I filaments. Shortening from initial sarcomere lengths at which the filaments overlapped took place through sliding of the filaments. If shortening was initiated from sarcomere lengths at which there was a gap, a narrowing of the I band was brought about by a curling of the I filaments at the boundary between the A and I bands. No evidence could be found that the I filaments moved into the A band.
DOI: 10.1083/jcb.19.2.369
发表时间: 1963-01-01
影响因子: 7.8
作者:
PAGE, SG;HUXLEY, HE
通讯作者: HUXLEY, HE
DOI: 10.1083/jcb.27.1.25
发表时间: 1965-10
期刊: The Journal of cell biology
影响因子: --
作者:
Carlsen F;Fuchs F;Knappeis GG
通讯作者: Knappeis GG
DOI: 10.1038/173971a0
发表时间: 1954-01-01
期刊: NATURE
影响因子: 64.8
作者:
HUXLEY, AF;NIEDERGERKE, R
通讯作者: NIEDERGERKE, R
DOI: 10.1016/s0022-2836(63)80081-9
发表时间: 1963-01-01
影响因子: 5.6
作者:
HANSON, J;LOWY, J
通讯作者: LOWY, J
DOI: 10.1083/jcb.11.1.95
发表时间: 1961-10
期刊: The Journal of biophysical and biochemical cytology
影响因子: --
作者:
CARLSEN F;KNAPPEIS GG;BUCHTHAL F
通讯作者: BUCHTHAL F