Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue
Dataset of Escherichia coli O157: H7 genes enriched in adherence to spinach root tissue
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大肠杆菌 O157 数据集：富含菠菜根组织粘附的 H7 基因
DOI：
10.1016/j.dib.2020.105769
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发表时间：
2020
期刊：
影响因子：
1.2
通讯作者：
Holmes A
中科院分区：
文献类型：
--
作者：
Holmes A
采用高通量正选择方法建立了志贺毒素大肠杆菌(STEC)O157：H7基因粘附性丰富的数据集。该方法基于在坚持之后在输出中丰富的BAC克隆与用作输入的接种相比较来生成差异数据集。从STEC分离株‘Sakai’衍生的BAC克隆文库被使用，因为该分离株与食用受污染的新鲜农产品-白萝卜芽-的人类疾病的大规模爆发有关。菠菜被用于筛选，因为它与STEC疫情有关，而菠菜的根为细菌定植提供了合适的地点。连续四轮Sakai BAC无性系选择和扩增用于菠菜的根部附着，并与非植物对照平行。从植物处理的细菌总量为7.17cfu× × 108cfu/ml，非植物对照的细菌总量为1.13cfu× ×109cfu/ml。使用已建立的E来获得与投入池相比的产出的相对基因丰度。STEC的ColiDNA微阵列芯片。该数据集能够筛选在处理条件下丰富的基因，并提供可能在植物与微生物相互作用中发挥作用的基因的信息。
A high-throughput positive-selection approach was taken to generate a dataset of ShigatoxigenicEscherichia coli(STEC) O157:H7 genes enriched in adherence to plant tissue. The approach generates a differential dataset based on BAC clones enriched in the output, after adherence, compared to the inoculum used as the input. A BAC clone library derived from STEC isolate ‘Sakai’ was used since this isolate is associated with a very large-scale outbreak of human disease from consumption of contaminated fresh produce; white radish sprouts. Spinach was used for the screen since it is associated with STEC outbreaks, and the roots provide a suitable site for bacterial colonisation. Four successive of rounds of Sakai BAC clone selection and amplification were applied for spinach root adherence, in parallel to a non-plant control. Genomic DNA was obtained from a total of 7.17 × 108cfu/ml of bacteria from the plant treatment and 1.13 × 109cfu/ml of bacteria from the no-plant control. Relative gene abundance of the output compared to the input pools was obtained using an establishedE. coliDNA microarray chip for STEC. The dataset enables screening for genes enriched under the treatment condition and informs on genes that may play a role in plant-microbe interactions.
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：用于比较 RNA-seq 和微阵列平台的 H7 转录组数据集
DOI：
--
发表时间：
2018
期刊：
影响因子：
--
作者：
Ewa Grabowiecka;David Martin;L. Crozier;N. Holden
通讯作者：
N. Holden
影响因子：
5.2
作者：
Bai J;McAteer SP;Paxton E;Mahajan A;Gally DL;Tree JJ
通讯作者：
Tree JJ
影响因子：
4.1
作者：
Hayashi, T;Makino, K;Shinagawa, H
通讯作者：
Shinagawa, H
影响因子：
5
作者：
Michino, H;Araki, K;Yanagawa, H
通讯作者：
Yanagawa, H