Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation.
Cytokine profiles of aqueous humor and graft in orthotopic mouse corneal transplantation.
复制标题
原位小鼠角膜移植中房水和移植物的细胞因子谱。
作者：
Satoru Yamagami;H. Kawashima;Hitoshi Endo;Tadahiko Tsuru;Hirohumi Shibui;Yasuo Kagawa;Junko Hori;Hiroko Yamagami;Mitsuaki Isobe
背景
细胞因子谱是了解同种异体移植排斥反应机制的关键。在角膜移植中，房水中细胞因子的表达以及房水细胞与角膜浸润细胞之间的相关性尚未完全了解。
方法
以BALB/c（H2 d）小鼠为受体，C3 H/He（H2k）和BALB/c小鼠为供体，分别进行同种异体和同种异体小鼠角膜移植。对房水细胞进行免疫细胞化学。对角膜移植进行了组织化学研究。用半定量逆转录聚合酶链反应方法检测房水和角膜移植片浸润细胞的细胞因子基因表达。
结果
干扰素-γ，白细胞介素（IL）-2，IL-4和IL-10的蛋白质和基因表达水平的细胞浸润的房水和角膜移植物中检测。然而，与Th 2细胞因子的表达相比，Th 1细胞因子在蛋白质水平的表达在排斥的同种异体移植物中始终占主导地位。细胞因子和表面标志物的配置文件中的细胞在房水中对应以及浸润角膜移植的细胞。房水中细胞因子蛋白和mRNA表达水平迅速下降。
结论
角膜移植中的同种异体排斥反应以Th 1型白细胞介素为主。与Th 1细胞因子相比，在移植排斥反应中，浸润细胞不表达Th 2细胞因子。房水细胞浸润模式与角膜细胞浸润模式具有良好的相关性。
BACKGROUND
Cytokine profile is a key in understanding the mechanisms of allograft rejection. Cytokine expression in the aqueous humor and the correlation between the aqueous humor cells and corneal infiltrating cells are not fully understood in corneal transplantation.
METHODS
Orthotopic mouse corneal transplantation was performed using BALB/c (H2d) mice as recipients, and C3H/He (H2k) and BALB/c mice as donors for allografts and isografts, respectively. Immunocytochemistry was performed on aqueous humor cells. Corneal graft was studied immunohistochemically. Cytokine gene expressions of the cells infiltrating the aqueous humor and corneal grafts were determined by the semiquantitative reverse transcription and polymerase chain reaction method.
RESULTS
Interferon-gamma, interleukin (IL)-2, IL-4, and IL-10 were detected in the cells infiltrating the aqueous humor and corneal grafts at both the protein and gene expression levels. T helper 1 (Th1) cytokine expressions at the protein level, however, were consistently predominant in the rejected allografts compared to those of Th2 cytokines. The cytokine and surface marker profiles of the cells in the aqueous humor corresponded well to those of the cells infiltrating the corneal grafts. Cytokine protein and mRNA expression levels in the aqueous humor decreased rapidly.
CONCLUSIONS
Allorejection in corneal transplantation is Th1 cytokine-predominant. Infiltrating cells do not express Th2 cytokine so much in allograft rejection, as compared with Th1 cytokine. The cell infiltration patterns of the aqueous humor were well correlated with those of the cornea.
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影响因子：
--
作者：
Niederkorn, J Y
通讯作者：
Niederkorn, J Y
小鼠原位角膜同种异体移植物引起的细胞介导的免疫反应的表征。
影响因子：
4.4
作者：
Sonoda,Y;Sano,Y;Ksander,B;Streilein,JW
通讯作者：
Streilein,JW
在同种异体肝移植受者和体外，IL-2 和 IL-5 基因表达对同种异体抗原的反应。
影响因子：
6.2
作者：
Martinez,OM;Villanueva,JC;Lake,J;Roberts,JP;Ascher,NL;Krams,SM
通讯作者：
Krams,SM
影响因子：
4.4
作者：
Andrew W. Taylor;J. Streilein;Scott W. Cousins
通讯作者：
Andrew W. Taylor;J. Streilein;Scott W. Cousins
用于定量鼠细胞因子 mRNA 分子的合成标准 DNA 构建体。
影响因子：
3.6
作者：
Farrar,JD;Street,NE
通讯作者：
Street,NE