Identification of a Novel TATA Element-binding Protein Binding Region at the N Terminus of the Saccharomyces cerevisiae TAF1 Protein*
Identification of a Novel TATA Element-binding Protein Binding Region at the N Terminus of the Saccharomyces cerevisiae TAF1 Protein*
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酿酒酵母 TAF1 蛋白 N 末端的新型 TATA 元件结合蛋白结合区的鉴定*
DOI：
10.1074/jbc.m306886200
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发表时间：
2003
影响因子：
4.8
通讯作者：
T. Kokubo
中科院分区：
文献类型：
--
作者：
Shinya Takahata;H. Ryu;Kazushige Ohtsuki;K. Kasahara;M. Kawaichi;T. Kokubo
TFIID是由TATA元件结合蛋白(TBP)和14个TBP相关因子(TAFs)组成的多蛋白复合体，可以直接识别核心启动子元件并介导转录激活。TAF1的TAF N末端结构域(TAND)可能通过调节TBP对TATA元件的访问而在这两个主要的TFIID功能中发挥重要作用。在酵母中，TAND由TAND1(10-37个氨基酸)和TAND2(46-71AA)两个亚区组成，分别与TBP的凹面和凸面相互作用。在这里，我们证明了位于TAND2(82-139氨基酸)C末端的另一个区域也可以与TBP结合，并作为GAL4融合蛋白与DNA结合并诱导转录激活。由于这些性质与TAND1的相同，我们将该序列表示为TAND3。详细的突变分析表明，位于TAND3内的三个疏水氨基酸残基不仅是TBP结合和转录激活所必需的，而且也是支持细胞生长和有效转录一组基因所必需的。我们还表明，TAND3识别的TBP表面比TAND1识别的TBP表面宽，尽管这些区域部分重叠。支持这些观察结果的是，TAND1至少在功能上可以被TAND3部分取代。
TFIID, a multiprotein complex composed of TATA element-binding protein (TBP) and 14 TBP-associated factors (TAFs), can directly recognize core promoter elements and mediate transcriptional activation. The TAF N-terminal domain (TAND) of TAF1 may play a significant role in these two principal TFIID functions by regulating the access of TBP to the TATA element. In yeast, TAND consists of two subdomains, TAND1 (10-37 amino acids (aa)) and TAND2 (46-71 aa), which interact with the concave and convex surfaces of TBP, respectively. Here we demonstrate that another region located on the C-terminal side of TAND2 (82-139 aa) can also bind to TBP and induce transcriptional activation when tethered to DNA as a GAL4 fusion protein. As these properties are the same as those of TAND1, we denoted this sequence as TAND3. Detailed mutational analyses revealed that three blocks of hydrophobic amino acid residues located within TAND3 are required not only for TBP binding and transcriptional activation but also for supporting cell growth and the efficient transcription of a subset of genes. We also show that the surface of TBP recognized by TAND3 is broader than that recognized by TAND1, although these regions overlap partially. Supporting these observations is that TAND1 can be at least partly functionally substituted by TAND3.
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DOI：
10.1073/pnas.94.3.820
发表时间：
1997-02-04
影响因子：
11.1
作者：
Kim, S;Na, JG;Reinberg, D
通讯作者：
Reinberg, D
影响因子：
10.5
作者：
AUBLE, DT;HANSEN, KE;HAHN, S
通讯作者：
HAHN, S
影响因子：
10.5
作者：
Bryant, GO;Martel, LS;Berk, AJ
通讯作者：
Berk, AJ
TBP-TFIIA 相互作用对体内酸性激活剂的反应。
DOI：
10.1126/science.7604282
发表时间：
1995
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Stargell,LA;Struhl,K
通讯作者：
Struhl,K
DOI：
10.1073/pnas.91.9.3520
发表时间：
1994-04-26
影响因子：
11.1
作者：
KOKUBO, T;YAMASHITA, S;NAKATANI, Y
通讯作者：
NAKATANI, Y